Centre de Transfert, I.N.S.A., 31077 Toulouse Cédex, and Laboratoire de Microbiologie, I.N.S.A., 69621 Villeurbanne Cédex, France.
Appl Environ Microbiol. 1986 Oct;52(4):812-8. doi: 10.1128/aem.52.4.812-818.1986.
Thirteen virulent phages and two temperate phages of two closely related bacterial species (Lactobacillus lactis and L. bulgaricus) were compared for their protein composition, their antigenic properties, their restriction endonuclease patterns, and their DNA homology. The immunoblotting studies and the DNA-DNA hybridizations showed that the phages could be differentiated into two groups. One group contained 2 temperate phages of L. bulgaricus and 11 virulent phages of L. lactis. Inside each group, at least two common proteins of identical sizes could be detected for each phage. These proteins were able to cross-react in immunoblotting experiments with an antiserum raised against one phage of the same group. Temperate phage DNAs showed partial homology with DNAs from some virulent phages. These homologies seem to be located on the region coding for the structural proteins since recombinant plasmids coding for one of the major phage proteins of one phage were able to hybridize with the DNAs from phages of the same group. These results suggest that temperate and virulent phages may be related to one another.
对来自两种密切相关的细菌（乳酸乳球菌和保加利亚乳杆菌）的 13 种烈性噬菌体和 2 种温和噬菌体的蛋白质组成、抗原特性、限制内切酶图谱和 DNA 同源性进行了比较。免疫印迹研究和 DNA-DNA 杂交表明，噬菌体可以分为两组。一组包含 2 种保加利亚乳杆菌温和噬菌体和 11 种乳酸乳球菌烈性噬菌体。在每组内，至少可以检测到每种噬菌体的两个相同大小的共同蛋白质。这些蛋白质在针对同一组中一种噬菌体的抗血清的免疫印迹实验中能够发生交叉反应。温和噬菌体 DNA 与一些烈性噬菌体的 DNA 具有部分同源性。这些同源性似乎位于编码结构蛋白的区域，因为编码一种噬菌体主要噬菌体蛋白的重组质粒能够与同一组噬菌体的 DNA 杂交。这些结果表明，温和噬菌体和烈性噬菌体可能彼此相关。
