利用时间分辨发光镧系纳米探针直接检测全血中的循环肿瘤细胞。
Direct Detection of Circulating Tumor Cells in Whole Blood Using Time-Resolved Luminescent Lanthanide Nanoprobes.
机构信息
State Key Laboratory of Structural Chemistry, CAS Key Laboratory of Design and Assembly of, Functional Nanostructures, Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, China.
Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
出版信息
Angew Chem Int Ed Engl. 2019 Aug 26;58(35):12195-12199. doi: 10.1002/anie.201907605. Epub 2019 Jul 25.
The detection of circulating tumor cells (CTCs) is crucial to early cancer diagnosis and the evaluation of cancer metastasis. However, it remains challenging due to the scarcity of CTCs in the blood. Herein, we report an ultrasensitive platform for the direct detection of CTCs using luminescent lanthanide nanoprobes. These were designed to recognize the epithelial cell adhesion molecules on cancer cells, allowing signal amplification through dissolution-enhanced time-resolved photoluminescence (TRPL) and the elimination of short-lived autofluorescence interference. This enabled the direct detection of blood breast-cancer cells with a limit of detection down to 1 cell/well of a 96-well plate. Moreover, blood CTCs (≥10 cells mL ) can be detected in cancer patients with a detection rate of 93.9 % (14/15 patients). We envision that this ultrasensitive detection platform with excellent practicality may provide an effective strategy for early cancer diagnosis and prognosis evaluation.
循环肿瘤细胞（CTC）的检测对于癌症的早期诊断和转移评估至关重要。然而，由于血液中 CTC 的数量稀少，因此仍然具有挑战性。在此，我们报告了一种使用发光镧系纳米探针直接检测 CTC 的超灵敏平台。这些探针被设计用来识别癌细胞上的上皮细胞黏附分子，通过溶解增强的时间分辨光致发光（TRPL）实现信号放大，并消除短寿命的自发荧光干扰。这使得可以直接检测血液中的乳腺癌细胞，检测限低至 96 孔板每孔 1 个细胞。此外，还可以检测到癌症患者血液中的 CTC（≥10 个细胞/mL），检测率为 93.9%（15 名患者中的 14 名）。我们设想，这种具有出色实用性的超灵敏检测平台可能为癌症的早期诊断和预后评估提供有效的策略。
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