Guo Long, Zhang Dan, Wang Lei, Xue Zijing, Guo Mei, Duan Li, Zheng Yuguang
Hebei University of Chinese Medicine, School of Pharmacy, Shijiazhuang, China, 050200.
Hebei Normal University, College of Chemistry and Material Science, Shijiazhuang, China, 050024.
J AOAC Int. 2019 Nov 1;102(6):1814-1821. doi: 10.5740/jaoacint.19-0080. Epub 2019 Jul 9.
and are two morphologically similar herbal medicines derived from the genus. Although the two herbs have been used as herbal medicines for a long time, studies on their phytochemicals and bioactive compositions are still limited, and no research has been devoted to compare the volatile compounds in and . To compare the volatile constituents in and and to explore chemical markers for discrimination and quality evaluation of the two herbal medicines. A GC-MS-based metabolomic approach was employed to compare and discriminate and from the aspect of volatile compounds. Multivariate statistical methods, including principal component analysis and orthogonal partial least-squares discriminate analysis, were applied to explore chemical markers for discrimination of the two herbal medicines. Thirty volatile compounds were identified, and the chemical profiles of volatile compounds in and were quite similar. Principal component analysis and orthogonal partial least-squares discrimination analysis results indicated that the two herbal medicines could be distinguished effectively from each other. Ten volatile compounds were selected as potential chemical markers for discrimination of the two herbal medicines. The GC-MS-based metabolomics could be an acceptable strategy for comparison and discrimination of and as well as authentication of herbal medicines derived from other closely related species. GC-MS based metabolomic approach was firstly applied to compare and discriminate Artemisia argyi and Artemisia lavandulifolia.
艾叶和野艾蒿是两种形态相似的蒿属草药。尽管这两种草药作为草药使用已久，但对其植物化学物质和生物活性成分的研究仍然有限，且尚未有研究致力于比较艾叶和野艾蒿中的挥发性化合物。为了比较艾叶和野艾蒿中的挥发性成分，并探索用于区分和质量评价这两种草药的化学标志物。采用基于气相色谱-质谱联用（GC-MS）的代谢组学方法从挥发性化合物方面对艾叶和野艾蒿进行比较和区分。应用包括主成分分析和正交偏最小二乘法判别分析在内的多元统计方法来探索区分这两种草药的化学标志物。共鉴定出30种挥发性化合物，艾叶和野艾蒿中挥发性化合物的化学图谱非常相似。主成分分析和正交偏最小二乘法判别分析结果表明，这两种草药能够有效地区分开来。选择了10种挥发性化合物作为区分这两种草药的潜在化学标志物。基于GC-MS的代谢组学可作为比较和区分艾叶和野艾蒿以及鉴定其他近缘物种来源草药的一种可接受策略。基于GC-MS的代谢组学方法首次应用于比较和区分艾叶和野艾蒿。
