Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Hebei University of Chinese Medicine, Shijiazhuang 050200, China.
Bethune International Peace Hospital, Shijiazhuang 050200, China.
J AOAC Int. 2021 May 21;104(2):515-525. doi: 10.1093/jaoacint/qsaa105.
Artemisiae argyi Folium (AF) has been used as herbal medicine and a food supplement in China and other Asian countries. There is no report about whether the different developmental stages can influence the bioactive compositions of AF.
To investigate the dynamic changes of bioactive constituents of AF collected at different developmental stages.
The volatile and nonvolatile bioactive components in AF collected at six different developmental stages were analyzed by gas chromatography-mass spectrometry and (GC-MS) high-performance liquid chromatography (HPLC). Chemometrics analysis, including principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA), were further performed to compare and discriminate the AF samples based on the analysis results.
The results indicated the bioactive compositions in AF underwent obvious changes during the period of growth. The volatile compositions of AF collected at different stages were the same, while their relative contents were different. Six volatile compounds could be regarded as chemical markers, which were responsible for the intergroup differences. The phenolic profiles of AF at different stages indicated a similar composition when the content levels of the main phenolic compounds were variated.
The comparative results will facilitate better understanding of dynamic changes of bioactive constituents of AF samples collected at different stages and will provide useful information for cultivation and utilization of this herbal medicine.
The volatile and nonvolatile bioactive components in AF collected at different developmental stages were evaluated and compared for the first time.
艾叶在中、日等亚洲国家被广泛用作草药和食品补充剂。然而，目前尚无报道探讨不同发育阶段对艾叶生物活性成分的影响。
本研究旨在考察不同发育阶段艾叶生物活性成分的动态变化。
采用气相色谱-质谱联用（GC-MS）和高效液相色谱（HPLC）对 6 个不同发育阶段的艾叶中的挥发性和非挥发性生物活性成分进行分析。基于分析结果，采用主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）等化学计量学方法对艾叶样品进行比较和判别。
结果表明，艾叶在生长过程中生物活性成分发生明显变化。不同发育阶段艾叶的挥发性成分相同，但相对含量不同。6 种挥发性化合物可作为化学标志物，其差异导致了组间差异。不同发育阶段艾叶的酚类成分谱表明，当主要酚类化合物的含量不同时，其组成相似。
本研究结果将有助于更好地理解不同发育阶段艾叶生物活性成分的动态变化，为该草药的栽培和利用提供有用信息。
本研究首次对不同发育阶段艾叶中的挥发性和非挥发性生物活性成分进行了评估和比较。
