State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, Jilin, China.
Key Lab for Molecular Enzymology & Engineering of Ministry of Education, Jilin University, Changchun, 130012, Jilin, China.
Anal Bioanal Chem. 2019 Sep;411(23):6021-6029. doi: 10.1007/s00216-019-01981-1. Epub 2019 Jul 9.
Cytobiological methods for cell nucleus-related studies start with the extraction processes of intranuclear components with many cell lysis buffers, following with the structural characterizations and quantitative analysis of the extracted components. In this study, we tried to evaluate the availability and reliability of these extraction-based analytical methods from their spectral features. We implemented an in situ surface-enhanced Raman scattering spectroscopy (SERS) strategy with the help of the nucleus-targeting nanoprobes to investigate the molecular information of nucleus, in comparison with these ex situ methods. This study provides valuable references for choosing an appropriate detection method according to different detection purposes, and also points out the risks of many developing cell-related analytical methods that combine the traditional cytobiological techniques from exogenous interferences during sample preprocesses. Graphical abstract.
用于细胞核相关研究的细胞生物学方法首先使用许多细胞裂解缓冲液进行核内成分的提取过程，然后对提取成分进行结构表征和定量分析。在这项研究中，我们试图从光谱特征评估这些基于提取的分析方法的可用性和可靠性。我们借助核靶向纳米探针实施了一种原位表面增强拉曼散射光谱 (SERS) 策略，以与这些非原位方法进行比较，来研究细胞核的分子信息。这项研究为根据不同的检测目的选择合适的检测方法提供了有价值的参考，同时也指出了许多将传统细胞生物学技术与样品预处理过程中外源干扰相结合的新兴细胞相关分析方法存在的风险。
