Zhang Jing, Liang Lijia, Guan Xin, Deng Rong, Qu Huixin, Huang Dianshuai, Xu Shuping, Liang Chongyang, Xu Weiqing
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun, Jilin, 130012, China.
Institute of Frontier Medical Science, Jilin University, Changchun, Jilin, 130021, China.
Anal Bioanal Chem. 2018 Jan;410(2):585-594. doi: 10.1007/s00216-017-0761-4. Epub 2017 Dec 6.
A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) probe (5-ethynyl-2'-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles. Graphical abstract A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS.
基于一种小型、生物相容性好、靶向细胞核的炔基标记脱氧核糖核酸（DNA）探针（5-乙炔基-2'-脱氧尿苷，EDU），开发了一种用于原位检测和分析细胞内核生物分子信息的表面增强拉曼散射（SERS）方法。该探针在DNA复制过程中可特异性地积聚在细胞核中，能够精确定位核区域，且不会干扰细胞的生物学活性和功能。由于特定的炔基在细胞的拉曼沉默区域显示出拉曼峰，因此在测量细胞的SERS光谱时，它是一种内部标记物，可实时同步可视化细胞核的位置。由于该方法未使用荧光标记染料来定位细胞核，所以它操作简单、无损、无光漂白现象，对于原位探索细胞器中涉及DNA的重要生理过程具有重要价值。图形摘要 开发了一种通用策略，通过SERS准确地定位核区域并获取细胞核的精确分子信息。
