Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea.
Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, South Korea.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1715-1723. doi: 10.1007/s10482-019-01300-9. Epub 2019 Jul 9.
A Gram-strain negative, aerobic, catalase and oxidase positive, non-motile, short rod-shaped bacterium, designated 17mud1-8, was isolated from mud collected from Nowon-gu, Seoul, South Korea. The strain was found to be able to grow at 10-40 °C (optimum 28-30 °C), pH 5.0-8.0 (optimum 7.0), and in the absence of NaCl. The nearly full-length 16S rRNA gene of strain 17mud1-8 exhibits sequence similarity of 94.1% with that of Panacibacter ginsenosidivorans Gsoil 1550, followed by 93.6% sequence similarity with Parafilimonas terrae DSM 28286. Phylogenetic analysis indicated that strain 17mud1-8 belongs to the family Chitinophagaceae, sharing approximately 94.1-91.9% sequence similarity with members of closely related genera. The respiratory quinone was identified as MK-7. The predominant fatty acids were found to consist of iso-C, iso-Cω5c and iso-C G. The polar lipids were identified as phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid, ten unidentified aminolipids and seven unidentified lipids. The draft genome of 17mud1-8 has G+C content of 40.9 mol% and a 5.8 Mb chromosome. On the basis of the phenotypic and genotypic properties, and phylogenetic inference, strain 17mud1-8 was found to represent a novel genus in the family Chitinophagaceae, for which the name Ilyomonas limi gen. nov., sp. nov. is proposed, with the type strain 17mud1-8(=KCTC 52874 = NBRC 112826).
一株革兰氏阴性、需氧、氧化酶和过氧化氢酶阳性、不运动、短杆状细菌，被命名为 17mud1-8，从韩国首尔瑞草区采集的泥浆中分离得到。该菌株能够在 10-40°C（最佳 28-30°C）、pH5.0-8.0（最佳 7.0）和无 NaCl 的条件下生长。菌株 17mud1-8 的近全长 16S rRNA 基因与 Panacibacter ginsenosidivorans Gsoil 1550 的序列相似性为 94.1%，其次与 Parafilimonas terrae DSM 28286 的序列相似性为 93.6%。系统发育分析表明，菌株 17mud1-8 属于噬几丁质菌科，与亲缘关系较近的属成员的序列相似性约为 94.1-91.9%。呼吸醌鉴定为 MK-7。主要脂肪酸由 iso-C、iso-Cω5c 和 iso-C G 组成。极性脂鉴定为磷脂酰乙醇胺、一种未鉴定的氨基磷脂、一种未鉴定的糖脂、十种未鉴定的氨基脂和七种未鉴定的脂。17mud1-8 的基因组草图 G+C 含量为 40.9mol%，染色体大小为 5.8Mb。基于表型和基因型特性以及系统发育推断，菌株 17mud1-8 代表噬几丁质菌科中的一个新属，命名为 Ilyomonas limi gen. nov.，sp. nov.，其模式菌株为 17mud1-8(=KCTC 52874 = NBRC 112826)。
