Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan.
Department of Plastic, Reconstructive and Aesthetic Surgery, Nippon Medical School, Tokyo, Japan.
Int J Dermatol. 2019 Dec;58(12):1398-1405. doi: 10.1111/ijd.14575. Epub 2019 Jul 9.
One suggested reason for aberrant wound healing in keloid scars is chronic inflammation of the dermis. We hypothesized that excessive blood vessel formation and high capillary density in keloid tissue is caused by dysfunction of endothelial progenitor cells.
We compared the number of circulating endothelial progenitor cells and vasculogenic and angiogenic capacity, as well as secretory function, of circulating CD34 cells in keloid patients and healthy individuals.
Compared to mononuclear cell cultures from healthy donors, cultures of peripheral blood mononuclear cells obtained from keloid patients showed a more than twofold increase in the number of peripheral blood EPCs (fibronectin-adhering cells that phagocytized acetylated low-density lipoprotein and bound Ulex europaeus agglutinin-I lectin). However, there was no difference in colony-forming ability and participation in in vitro angiogenesis between circulating CD34 cells isolated from keloid patients and healthy individuals. This means that circulating CD34 /endothelial progenitor cells in keloid patients have normal vasculogenic and angiogenic function. However, CD34 cells derived from keloid patients demonstrated a more than sevenfold expression of the interleukin-8 gene and a more than fivefold expression of the vascular endothelial growth factor gene than CD34 cells derived from healthy individuals.
These results support the role of vascular endothelial growth factor and interleukin-8 in increased recruitment of endothelial progenitor cells in keloid patients.
瘢痕疙瘩愈合异常的一个原因是真皮的慢性炎症。我们假设，瘢痕疙瘩组织中过多的血管形成和高毛细血管密度是由内皮祖细胞功能障碍引起的。
我们比较了瘢痕疙瘩患者和健康个体循环内皮祖细胞的数量以及血管生成和血管生成能力，以及循环 CD34 细胞的分泌功能。
与来自健康供体的单核细胞培养物相比，来自瘢痕疙瘩患者的外周血单核细胞培养物中的外周血 EPC（吞噬乙酰化低密度脂蛋白并结合荆豆凝集素-I 凝集素的纤维连接蛋白黏附细胞）数量增加了两倍以上。然而，从瘢痕疙瘩患者和健康个体分离的循环 CD34 细胞在体外血管生成中的集落形成能力和参与无差异。这意味着瘢痕疙瘩患者的循环 CD34/内皮祖细胞具有正常的血管生成和血管生成功能。然而，与来自健康个体的 CD34 细胞相比，来自瘢痕疙瘩患者的 CD34 细胞中白细胞介素 8 基因的表达增加了七倍以上，血管内皮生长因子基因的表达增加了五倍以上。
这些结果支持血管内皮生长因子和白细胞介素 8 在瘢痕疙瘩患者中内皮祖细胞募集增加中的作用。
