Mass Spectrometry Centre, Department of Chemistry and QOPNA , University of Aveiro , Campus Universitário de Santiago , 3810-193 Aveiro , Portugal.
Clinical Pathology , Centro Hospitalar do Baixo Vouga , Aveiro , Portugal.
J Proteome Res. 2019 Aug 2;18(8):3174-3183. doi: 10.1021/acs.jproteome.9b00249. Epub 2019 Jul 22.
Obesity is a public health problem and a risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, and nonalcoholic fatty liver disease. Given these clinical implications, there is a growing interest to understand the pathophysiological mechanism of obesity. Changes in lipid metabolism have been associated with obesity and obesity-related complications. However, changes in the lipid profile of obese children have been overlooked. In the present work, we analyzed the serum phospholipidome of overweight and obese children by HILIC-MS/MS and GC-MS. Using this approach, we have identified 165 lipid species belonging to the classes PC, PE, PS, PG, PI, LPC, and SM. The phospholipidome of overweight (OW) and obese (OB) children was significantly different from normal-weight children (control). Main differences were observed in the PI class that was less abundant in OW and OB children and some PS, PE, SM, and PC lipid species are upregulated in obese and overweight children. Although further studies are needed to clarify some association between phospholipid alterations and metabolic changes, our results highlight the alteration that occurs in the serum phospholipid profile in obesity in children.
肥胖是一个公共卫生问题，也是 2 型糖尿病、心血管疾病和非酒精性脂肪肝等疾病的一个风险因素。鉴于这些临床意义，人们越来越有兴趣了解肥胖的病理生理机制。脂质代谢的变化与肥胖和肥胖相关并发症有关。然而，肥胖儿童的脂质谱变化却被忽视了。在本工作中，我们通过 HILIC-MS/MS 和 GC-MS 分析了超重和肥胖儿童的血清磷脂组。通过这种方法，我们鉴定了属于 PC、PE、PS、PG、PI、LPC 和 SM 类的 165 种脂质。超重（OW）和肥胖（OB）儿童的磷脂组与正常体重儿童（对照组）有显著差异。PI 类的差异最为明显，OW 和 OB 儿童的 PI 类含量较少，一些 PS、PE、SM 和 PC 脂质的含量在肥胖和超重儿童中上调。虽然还需要进一步的研究来阐明磷脂变化与代谢变化之间的某些关联，但我们的结果强调了肥胖儿童血清磷脂谱的改变。
