Moore Aaron I, Moreira Ana S P, Guerra Inês M S, Goracci Laura, Domingues Pedro, Melo Tânia, Domingues M Rosário, O'Boyle Niamh M
School of Pharmacy and Pharmaceutical Sciences, Panoz Institute and Trinity Biomedical Sciences Institute, Trinity College Dublin, The University of Dublin, Dublin, Ireland.
CESAM-Centre for Environmental and Marine Studies, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
Contact Dermatitis. 2025 Mar;92(3):176-186. doi: 10.1111/cod.14711. Epub 2024 Oct 15.
Limonene and linalool are used in cosmetic products for their floral scents, but their oxidation products are strong contact allergens whose mechanisms of action are still not fully understood.
The effects of limonene hydroperoxide (Lim-2-OOH) and linalool hydroperoxides (Lin-6/7-OOH) on the lipid profile of a human keratinocyte cell line (HaCaT) were evaluated. 2,4-Dinitrofluorobenzene (DNFB) was also included.
Lim-2-OOH and Lin-6/7-OOH were synthesised according to previous methods. HaCaT cells were treated with allergens (10 μM) for 24 h and the cellular lipid extracts were analysed by C18 liquid chromatography with tandem mass spectrometry (LC-MS/MS). Data analysis was performed using Lipostar software. Statistical analysis was carried out using Metaboanalyst and R software.
All three sensitisers used caused significant changes in the lipidome of HaCaT cells in a similar trend. There was an upregulation in several plasmanyl/plasmenyl phospholipids (O-/P-phosphatidylcholines [PC] and O-/P-phosphatidylethanolamines [PE]), sphingolipids (HexCer) and triacylglycerol lipid species, and a decrease in some polyunsaturated fatty acids-containing phospholipid (PE and PC) species suggesting oxidative stress and inflammation.
This study is the first to evaluate the plasticity of the HaCaT cell lipidome in response to allylic hydroperoxide allergens Lim-2-OOH and Lin-6/7-OOH, together with the experimental contact allergen DNFB. These allergens are able to upregulate and downregulate certain lipid classes to a varying degree.
柠檬烯和芳樟醇因其花香气味而被用于化妆品中，但其氧化产物是强接触性过敏原，其作用机制仍未完全明确。
评估氢过氧化柠檬烯（Lim-2-OOH）和氢过氧化芳樟醇（Lin-6/7-OOH）对人角质形成细胞系（HaCaT）脂质谱的影响。同时纳入2,4-二硝基氟苯（DNFB）进行研究。
按照先前方法合成Lim-2-OOH和Lin-6/7-OOH。用过敏原（10 μM）处理HaCaT细胞24小时，然后通过C18液相色谱-串联质谱（LC-MS/MS）分析细胞脂质提取物。使用Lipostar软件进行数据分析。使用Metaboanalyst和R软件进行统计分析。
所使用的三种致敏剂均以相似趋势引起HaCaT细胞脂质组的显著变化。几种缩醛磷脂/缩醛磷脂酰胆碱（O-/P-磷脂酰胆碱[PC]和O-/P-磷脂酰乙醇胺[PE]）、鞘脂（己糖神经酰胺）和三酰甘油脂质种类上调，而一些含多不饱和脂肪酸的磷脂（PE和PC）种类减少，提示存在氧化应激和炎症。
本研究首次评估了HaCaT细胞脂质组对烯丙基氢过氧化物过敏原Lim-2-OOH和Lin-6/7-OOH以及实验性接触性过敏原DNFB的可塑性反应。这些过敏原能够不同程度地上调或下调某些脂质类别。
