Department of Pharmacology, College of basic Medicine, Qingdao University, Qingdao 266071, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, China.
Mar Drugs. 2019 Sep 17;17(9):540. doi: 10.3390/md17090540.
Obesity and its related complications have become one of the leading problems affecting human health. However, current anti-obesity treatments are limited by high cost and numerous adverse effects. In this study, we investigated the use of a non-toxic green food additive, known as unsaturated alginate oligosaccharides (UAOS) from the enzymatic degradation of Laminaria japonicais, which showed effective anti-obesity effects in a high-fat diet (HFD) mouse model. Compared with acid hydrolyzed saturated alginate oligosaccharides (SAOS), UAOS significantly reduced body weight, serum lipid, including triacylglycerol (TG), total cholesterol (TC) and free fatty acids (FFA), liver weight, liver TG and TC, serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels, adipose mass, reactive oxygen species (ROS) formation, and accumulation induced in HFD mice. Moreover, the structural differences in β-d-mannuronate (M) and its C5 epimer α-l-guluronate (G) did not cause significant functional differences. Meanwhile, UAOS significantly increased both AMP-activated protein kinase α (AMPKα) and acetyl-CoA carboxylase (ACC) phosphorylation in adipocytes, which indicated that UAOS had an anti-obesity effect mainly through AMPK signaling. Our results indicate that UAOS has the potential for further development as an adjuvant treatment for many metabolic diseases such as fatty liver, hypertriglyceridemia, and possibly diabetes.
肥胖及其相关并发症已成为影响人类健康的主要问题之一。然而，目前的抗肥胖治疗方法受到成本高和不良反应多的限制。在这项研究中，我们研究了一种无毒的绿色食品添加剂，即来自褐藻的不饱和藻酸盐寡糖（UAOS），它在高脂肪饮食（HFD）小鼠模型中显示出有效的抗肥胖作用。与酸水解的饱和藻酸盐寡糖（SAOS）相比，UAOS 显著降低了体重、血清脂质，包括三酰甘油（TG）、总胆固醇（TC）和游离脂肪酸（FFA）、肝重、肝 TG 和 TC、血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平、脂肪质量、HFD 小鼠中诱导的活性氧（ROS）形成和积累。此外，β-d-甘露糖（M）及其 C5 差向异构体α-l-古洛糖（G）的结构差异并没有引起明显的功能差异。同时，UAOS 显著增加了脂肪细胞中 AMP 激活的蛋白激酶α（AMPKα）和乙酰辅酶 A 羧化酶（ACC）的磷酸化，这表明 UAOS 主要通过 AMPK 信号通路发挥抗肥胖作用。我们的结果表明，UAOS 有潜力进一步开发为辅助治疗许多代谢疾病，如脂肪肝、高甘油三酯血症，甚至糖尿病。
