Wang Lining, Wu Xiangli, Gao Wei, Zhao Mengran, Zhang Jinxia, Huang Chenyang
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China.
Genes (Basel). 2017 Nov 21;8(11):335. doi: 10.3390/genes8110335.
Catalases are ubiquitous hydrogen peroxide-detoxifying enzymes. They participate in fungal growth and development, such as mycelial growth and cellular differentiation, and in protecting fungi from oxidative damage under stressful conditions. To investigate the potential functions of catalases in , we obtained two catalase genes from a draft genome sequence of , and cloned and characterized them ( and ). (group II) and (group III) encoded putative peptides of 745 and 528 amino acids, respectively. Furthermore, the gene structures were variant between and . Further research revealed that these two catalase genes have divergent expression patterns during different developmental stages. /Po-cat1 was at a barely detectable level in mycelia, accumulated gradually during reproductive growth, and was maximal in separated spores. But no catalase activity of Po-cat1 was detected by native-PAGE during any part of the developmental stages. In contrast, high /Po-cat2 expression and Po-cat2 activity found in mycelia were gradually lost during reproductive growth, and at a minimal level in separated spores. In addition, these two genes responded differentially under 32 °C and 40 °C heat stresses. was up-regulated under both temperature conditions, while was up-regulated at 32 °C but down-regulated at 40 °C. The accumulation of catalase proteins correlated with gene expression. These results indicate that the two divergent catalases in may play different roles during development and under heat stress.
过氧化氢酶是普遍存在的过氧化氢解毒酶。它们参与真菌的生长和发育，如菌丝体生长和细胞分化，并在应激条件下保护真菌免受氧化损伤。为了研究过氧化氢酶在[具体物种名称未给出]中的潜在功能，我们从[具体物种名称未给出]的基因组草图序列中获得了两个过氧化氢酶基因，并对它们进行了克隆和表征（[基因名称1]和[基因名称2]）。[基因名称1]（第二组）和[基因名称2]（第三组）分别编码推定的745和528个氨基酸的肽。此外，[基因名称1]和[基因名称2]之间的基因结构存在差异。进一步的研究表明，这两个过氧化氢酶基因在不同发育阶段具有不同的表达模式。/Po-cat1在菌丝体中的水平几乎检测不到，在生殖生长过程中逐渐积累，在分离的孢子中达到最大值。但在发育阶段的任何时期，通过非变性聚丙烯酰胺凝胶电泳均未检测到Po-cat1的过氧化氢酶活性。相比之下，在菌丝体中发现的高/Po-cat2表达和Po-cat2活性在生殖生长过程中逐渐丧失，在分离的孢子中处于最低水平。此外，这两个基因在32℃和40℃热胁迫下的反应不同。[基因名称1]在两种温度条件下均上调，而[基因名称2]在32℃时上调，但在40℃时下调。过氧化氢酶蛋白的积累与基因表达相关。这些结果表明，[具体物种名称未给出]中的两种不同的过氧化氢酶在发育和热胁迫下可能发挥不同的作用。
