1 Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Republic of Korea.
2 Department of Otorhinolaryngology Head and Neck Surgery, Sanggye Paik Hospital, Seoul, Republic of Korea.
J Appl Biomater Funct Mater. 2019 Jul-Sep;17(3):2280800019834520. doi: 10.1177/2280800019834520.
Hard palate, developed by embryo neural crest stem cells, is a tissue with strong regenerative abilities. It is considered an abundant source of progenitor cells, forming various mesenchymal tissues. Rabbits are more suitable models than murine animals for regenerative preclinical study of the head and neck, owing to their larger size. However, there are no reports of the existence or characteristics of neural crest stem cells in the hard palate of rabbits. In this study, we demonstrate for the first time the presence of nestin-, Sox2-, and p75-positive neural crest stem cells obtained from the hard palate of rabbits and the properties of these cells. Flow cytometry analysis revealed that CD29, CD44, and CD81 were positive; and CD11b, CD34, and CD90 were negative on the expanded palatal progenitor cells. Finally, we differentiated them into cells of mesenchymal lineages (bone, cartilage, and fat) , and in three-dimensional fabricated polycaprolactone and polycaprolactone-tricalcium phosphate scaffolds. Taken together, our data showed the existence of rabbit palatum-derived mesenchymal progenitor cells, and successful fabrication of progenitor cell-loaded biodegradable scaffold using three-dimensional printing. This study will open avenues for new tissue engineering strategies for cell therapy using three-dimensional printing with scaffolds for reconstruction of head and neck defects.
硬腭由胚胎神经嵴干细胞发育而来，是一种具有强大再生能力的组织。它被认为是祖细胞的丰富来源，可形成各种间充质组织。由于体型较大，兔子比鼠类动物更适合头颈部再生临床前研究的模型。然而，目前尚无关于兔子硬腭中存在神经嵴干细胞及其特性的报道。在这项研究中，我们首次证明了从兔子硬腭中获得的巢蛋白阳性、Sox2 阳性和 p75 阳性神经嵴干细胞的存在，并研究了这些细胞的特性。流式细胞术分析显示，扩增的腭祖细胞上 CD29、CD44 和 CD81 呈阳性，而 CD11b、CD34 和 CD90 呈阴性。最后，我们将它们分化为间充质谱系的细胞（骨、软骨和脂肪），并在三维聚己内酯和聚己内酯-磷酸三钙支架上进行了构建。综上所述，我们的数据表明了兔腭源性间充质祖细胞的存在，以及使用三维打印成功构建了祖细胞负载的可生物降解支架。这项研究将为使用三维打印支架进行头颈部缺陷重建的细胞治疗开辟新的组织工程策略。
