Allan G M, McNeilly F, Kennedy S, Daft B, Clarke E G, Ellis J A, Haines D M, Meehan B M, Adair B M
Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Belfast.
J Vet Diagn Invest. 1998 Jan;10(1):3-10. doi: 10.1177/104063879801000102.
Samples of lung, liver, kidney, pancreas, spleen, and lymph node from pigs with postweaning multisystemic wasting syndrome from California (USA) and samples of mesenteric lymph nodes from similarly diseased pigs from Brittany (France) were examined by light microscopy, in situ hybridization (ISH), and/or virus isolation. Whole genomic probes for porcine circovirus (PCV) and chicken anemia virus (CAV) were used for ISH. Tissue homogenate supernatants were inoculated onto PK/15 cells for virus isolation, and the presence of viral antigen and viral particles was verified by indirect immunofluorescence, ISH, and electron microscopy. Histologic examination of lung from pigs from California revealed interstitial pneumonia, alveolar epithelial hyperplasia, and basophilic nuclear and cytoplasmic inclusions in mononuclear cell infiltrates and various pulmonary epithelial cells. Granulomatous lymphadenitis with syncytial cells typified the lesions seen in the pigs from France. PCV-like nucleic acid was detected by ISH in lung, pancreas, lymph node, kidney, and liver in pigs from California. Positive signal was also obtained in lymph node sections from pigs from France. Probes for CAV were consistently negative. PK/15 cell cultures inoculated with lung preparations from diseased California pigs and mesenteric lymph node preparations from pigs from France had positive fluorescence by indirect staining for PCV using pooled polyclonal pig sera and hyperimmune rabbit serum and had variable staining with a panel of 7 monoclonal antibodies specific for cell culture contaminant PCV. PCV-like nucleic acid was also detected by ISH in cell cultures. Cytopathic effect was not observed. Electron microscopic examination of inoculated cell cultures revealed 17-nm viral particles morphologically consistent with PCV. No other virus particles were observed. Although genomic analysis for the definitive identification of these viral isolates remains to be done, the evidence provided strongly suggests that these tissue isolates are closely related to, although antigenically distinct from, the original PCV cell culture contaminant.
对来自美国加利福尼亚州患有断奶后多系统消瘦综合征的猪的肺、肝、肾、胰腺、脾和淋巴结样本，以及来自法国布列塔尼患有类似疾病的猪的肠系膜淋巴结样本进行了光学显微镜检查、原位杂交（ISH）和/或病毒分离。使用猪圆环病毒（PCV）和鸡贫血病毒（CAV）的全基因组探针进行ISH。将组织匀浆上清液接种到PK/15细胞上进行病毒分离，并通过间接免疫荧光、ISH和电子显微镜检查来验证病毒抗原和病毒颗粒的存在。对来自加利福尼亚州的猪的肺进行组织学检查发现有间质性肺炎、肺泡上皮增生，以及单核细胞浸润和各种肺上皮细胞中的嗜碱性核内和胞质内包涵体。伴有多核巨细胞的肉芽肿性淋巴结炎是法国猪所见到病变的典型特征。通过ISH在来自加利福尼亚州的猪的肺、胰腺、淋巴结、肾和肝中检测到了类似PCV的核酸。在来自法国的猪的淋巴结切片中也获得了阳性信号。CAV探针始终为阴性。用来自患病的加利福尼亚州猪的肺制剂和来自法国的猪的肠系膜淋巴结制剂接种的PK/15细胞培养物，使用混合的多克隆猪血清和超免疫兔血清进行间接染色时，对PCV呈阳性荧光，并且用一组针对细胞培养污染物PCV的7种单克隆抗体染色结果不一。在细胞培养物中也通过ISH检测到了类似PCV的核酸。未观察到细胞病变效应。对接种的细胞培养物进行电子显微镜检查发现了形态上与PCV一致的17纳米病毒颗粒。未观察到其他病毒颗粒。尽管对这些病毒分离株进行最终鉴定的基因组分析仍有待完成，但所提供的证据强烈表明，这些组织分离株与原始的PCV细胞培养污染物密切相关，尽管在抗原性上有所不同。
