Chulabhorn International College of Medicine, Thammasat University, Rangsit Campus , Pathum Thani, 12120, Thailand.
Present address: Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
BMC Complement Altern Med. 2019 Jul 10;19(1):168. doi: 10.1186/s12906-019-2535-3.
Several immunological pathways, particularly skin inflammation via various pro-inflammatory cytokines have been reported to be involved in the pathogenesis and clinical manifestations of psoriasis. The aim of the study was to investigate the potential role of naringin from Citrus maxima (Burm.) Merr and sericin from Bombyx mori combination in the treatment of psoriasis. Inhibitory effects on the expression of mRNA and the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-23, and IL-12p40) were investigated.
Human peripheral blood mononuclear cells (hPBMCs) were isolated from 10 healthy subjects and 10 patients with psoriasis. The hPBMCs from each group were exposed to naringin or sericin alone, and the combination of naringin and sericin. The expression levels of mRNA and the production of all cytokines were determined using quantitative RT-PCR and ELISA, respectively.
Naringin/sericin combination significantly decreased the expression of mRNA and the production of all pro-inflammatory cytokines in hPBMCs from patients with psoriasis. The potency of inhibitory activity was markedly higher than naringin or sericin alone.
The activity of naringin/sericin combination on down-regulation of these pro-inflammatory cytokines suggested its potential clinical use in psoriasis as well as other inflammation-associated diseases. The combination might be used as a complementary therapy with conventional treatment in psoriasis to improve clinical efficacy and tolerability.
几种免疫途径，特别是通过各种促炎细胞因子引起的皮肤炎症，已被报道与银屑病的发病机制和临床表现有关。本研究旨在探讨柚皮苷（Citrus maxima（Burm.） Merr）和丝胶（Bombyx mori）联合应用对银屑病治疗的潜在作用。研究了其对 mRNA 表达和促炎细胞因子（TNF-α、IL-6、IL-23 和 IL-12p40）产生的抑制作用。
从 10 名健康受试者和 10 名银屑病患者中分离人外周血单个核细胞（hPBMCs）。每组 hPBMCs 分别暴露于柚皮苷或丝胶单独，以及柚皮苷和丝胶的组合中。使用定量 RT-PCR 和 ELISA 分别测定 mRNA 的表达水平和所有细胞因子的产生。
柚皮苷/丝胶联合显著降低了银屑病患者 hPBMCs 中所有促炎细胞因子的 mRNA 表达和产生。抑制活性的效力明显高于柚皮苷或丝胶单独使用。
柚皮苷/丝胶联合下调这些促炎细胞因子的活性表明其在银屑病以及其他炎症相关疾病中的潜在临床应用。该联合可能被用作银屑病常规治疗的补充治疗方法，以提高临床疗效和耐受性。
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