Horii Kazuhiro, Suzuki Yuji, Shiina Takahiko, Saito Shouichiro, Onouchi Sawa, Horii Yuuki, Shimaoka Hiroki, Shimizu Yasutake
Department of Basic Veterinary Science, Laboratory of Physiology, The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagaido, Gifu 501-1193, Japan.
Department of Basic Veterinary Science, Laboratory of Anatomy, The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagaido, Gifu 501-1193, Japan.
J Vet Med Sci. 2019 Sep 3;81(9):1266-1272. doi: 10.1292/jvms.19-0197. Epub 2019 Jul 9.
The aim of the present study was to clarify roles of ATP-dependent potassium channels (K channels) in motility of the striated muscle portion in the esophagus. An isolated segment of the rat esophagus was placed in an organ bath and mechanical responses were recorded using a force transducer. Electrical stimulation of the vagus nerve evoked contractile response of striated muscle in the esophageal segment. Application of glibenclamide, an antagonist of K channels, increased amplitude of vagally mediated twitch contractions of the rat esophagus. On the other hand, minoxidil, an agonist of K channels, decreased amplitude of twitch contractions. RT-PCR revealed the expression of subunits of K channels in esophageal tissue. In addition, immunopositivity for subunits of K channels was observed in the striated muscle cells of the esophageal muscle layer. These findings indicate that K channels contribute to motor regulation of striated muscle in the rat esophagus.
本研究的目的是阐明ATP依赖性钾通道（K通道）在食管横纹肌部分运动中的作用。将大鼠食管的一个分离段置于器官浴槽中，使用力传感器记录机械反应。迷走神经的电刺激诱发食管段横纹肌的收缩反应。应用K通道拮抗剂格列本脲可增加大鼠食管迷走神经介导的抽搐收缩幅度。另一方面，K通道激动剂米诺地尔可降低抽搐收缩幅度。逆转录聚合酶链反应（RT-PCR）显示食管组织中存在K通道亚基的表达。此外，在食管肌层的横纹肌细胞中观察到K通道亚基的免疫阳性。这些发现表明K通道有助于大鼠食管横纹肌的运动调节。
