Department of Cardiovascular Surgery, Division of Experimental Surgery, Institute Insure (Institute for Translational Cardiac Surgery), German Heart Center Munich at the Technical University of Munich, Munich, Germany.
Department of Thoracic and Cardiovascular Surgery, Herz- und Diabeteszentrum NRW, University Hospital Ruhr-University Bochum, Bad Oeynhausen, Germany.
Sci Rep. 2019 Jul 10;9(1):9986. doi: 10.1038/s41598-019-46123-w.
Myosin binding protein H-like (MYBPHL) is a protein associated with myofilament structures in atrial tissue. The protein exists in two isoforms that share an identical amino acid sequence except for a deletion of 23 amino acids in isoform 2. In this study, MYBPHL was found to be expressed preferentially in atrial tissue. The expression of isoform 2 was almost exclusively restricted to the atria and barely detectable in the ventricle, arteria mammaria interna, and skeletal muscle. After atrial damage induced by cryo- or radiofrequency ablation, MYBPHL was rapidly and specifically released into the peripheral circulation in a time-dependent manner. The plasma MYBPHL concentration remained substantially elevated up to 24 hours after the arrival of patients at the intensive care unit. In addition, the recorded MYBPHL values were strongly correlated with those of the established biomarker CK-MB. In contrast, an increase in MYBPHL levels was not evident in patients undergoing aortic valve replacement or transcatheter aortic valve implantation. In these patients, the values remained virtually constant and never exceeded the concentration in the plasma of healthy controls. Our findings suggest that MYBPHL can be used as a precise and reliable biomarker to specifically predict atrial myocardial damage.
肌球蛋白结合蛋白 H 样蛋白 (MYBPHL) 是一种与心房组织中的肌丝结构相关的蛋白。该蛋白存在两种同工型，它们的氨基酸序列完全相同，只是同工型 2 中缺失了 23 个氨基酸。在这项研究中，发现 MYBPHL 优先在心房组织中表达。同工型 2 的表达几乎完全局限于心房，在心室、乳内动脉和骨骼肌中几乎检测不到。在冷冻或射频消融诱导心房损伤后，MYBPHL 迅速且特异性地以时间依赖性方式释放到外周循环中。在重症监护病房到达患者后，血浆 MYBPHL 浓度持续升高，高达 24 小时。此外，记录的 MYBPHL 值与已建立的生物标志物 CK-MB 强烈相关。相比之下，在接受主动脉瓣置换或经导管主动脉瓣植入术的患者中，MYBPHL 水平的增加并不明显。在这些患者中，值几乎保持不变，从未超过健康对照组血浆中的浓度。我们的研究结果表明，MYBPHL 可用作一种精确和可靠的生物标志物，专门预测心房心肌损伤。
