Department of Speech, Language and Hearing, University of Texas at Dallas, Dallas, Texas, U.S.A.
Department of Otolaryngology-Head and Neck Surgery, University of Texas at Southwestern Medical Center, Dallas, Texas, U.S.A.
Laryngoscope. 2022 Jan;132(1):148-155. doi: 10.1002/lary.29683. Epub 2021 Jun 11.
The goals of this study were to 1) compare global protein expression in muscles of the larynx and hindlimb and 2) investigate differences in protein expression between aged and nonaged muscle using label-free global proteomic profiling methods.
Liquid chromatography-mass spectrometry (LC-MS/MS) analysis was performed on thyroarytenoid intrinsic laryngeal muscle and plantaris hindlimb muscle from 10 F344xBN F1 male rats (5 old and 5 young). Protein expression was compared and pathway enrichment analysis performed for each muscle type (larynx and limb) and age group (old and young muscle).
Over 1,000 proteins were identified in common across both muscle types and age groups using LC-MS/MS analysis. Significant age-related differences were seen across 107 proteins in plantaris hindlimb and in 19 proteins in thyroarytenoid laryngeal muscle. Bioinformatic and enrichment analysis demonstrated protein differences between the hindlimb and larynx may relate to immune and stress redox responses and RNA repair.
There are clear differences in protein expressions between the laryngeal and hindlimb skeletal muscles. Initial analysis suggests differences between the two muscle groups may relate to stress responses and repair mechanisms. Age-related changes in the thyroarytenoid appear to be less obvious than in the plantaris. Further in-depth study is needed to elucidate how aging affects protein expression in the laryngeal muscles.
NA Laryngoscope, 132:148-155, 2022.
本研究旨在：1）比较喉和后肢肌肉的整体蛋白质表达，2）利用非标记整体蛋白质组学分析方法研究不同年龄肌肉之间的蛋白表达差异。
采用液相色谱-质谱（LC-MS/MS）分析技术，对 10 只 F344xBN F1 雄性大鼠（5 只老年和 5 只年轻）的杓状软骨内收肌和比目鱼肌进行检测。对每种肌肉类型（喉和肢体）和年龄组（老年和年轻肌肉）进行蛋白质表达比较和途径富集分析。
通过 LC-MS/MS 分析，在两种肌肉类型和年龄组中共同鉴定出超过 1000 种蛋白质。在比目鱼肌中，有 107 种蛋白与年龄相关，在杓状软骨喉肌中有 19 种蛋白存在显著差异。生物信息学和富集分析表明，后肢和喉之间的蛋白质差异可能与免疫和应激氧化还原反应及 RNA 修复有关。
喉和后肢骨骼肌的蛋白表达存在明显差异。初步分析表明，两组肌肉之间的差异可能与应激反应和修复机制有关。与比目鱼肌相比，杓状软骨的年龄相关性变化不那么明显。需要进一步深入研究，阐明衰老如何影响喉肌的蛋白表达。
无 喉科杂志，132：148-155，2022。
