Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Chaoyang District, Beijing, China.
Food Funct. 2019 Aug 1;10(8):4661-4673. doi: 10.1039/c9fo00896a. Epub 2019 Jul 11.
Hydroxysafflor yellow A (HSYA) is the main active ingredient of edible plant safflower. HSYA has demonstrated anti-inflammatory effects. The inflammatory response is the key mechanism responsible for asthma, and the pro-inflammatory platelet-activating factor (PAF) is known to play a role in the pathology of bronchial asthma. In this study, we stimulated human bronchial smooth muscle cells (HBSMCs) with PAF and examined the effects of HSYA on the resulting asthma-related process. PAF stimulation induced HBSMC activation, induced proliferation, increased expression of the pro-inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor-α, and activated asthma-related signaling pathways. All these effects were significantly inhibited by treatment with HSYA (9, 27, 81 μmol L). The effects of HSYA were prevented by the addition of a PAF receptor (PAFR) antagonist or by PAFR gene silencing with small interfering RNA. These results suggest that HSYA may inhibit PAF-induced activation of HBSMCs by targeting the PAFR. Overall, these findings provide evidence that HSYA can be applied as a potential therapeutic agent in the treatment of bronchial asthma.
羟基红花黄色素 A（HSYA）是食用植物红花的主要活性成分。HSYA 具有抗炎作用。炎症反应是哮喘的关键机制，已知促炎血小板活化因子（PAF）在支气管哮喘的发病机制中起作用。在这项研究中，我们用 PAF 刺激人支气管平滑肌细胞（HBSMC），并研究 HSYA 对由此产生的与哮喘相关的过程的影响。PAF 刺激诱导 HBSMC 激活，诱导增殖，增加促炎细胞因子白细胞介素（IL）-6、IL-1β 和肿瘤坏死因子-α的表达，并激活与哮喘相关的信号通路。所有这些作用均被 HSYA（9、27、81 μmol L）处理显著抑制。用 PAFR 拮抗剂或用小干扰 RNA 沉默 PAFR 基因处理可预防 HSYA 的作用。这些结果表明，HSYA 可能通过靶向 PAFR 抑制 PAF 诱导的 HBSMC 激活。总的来说，这些发现为 HSYA 可作为治疗支气管哮喘的潜在治疗剂提供了证据。
