Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Beijing, People's Republic of China.
School of Life Sciences, Jilin University, Changchun, 130012, Jilin, People's Republic of China.
Curr Microbiol. 2019 Dec;76(12):1387-1397. doi: 10.1007/s00284-019-01739-z. Epub 2019 Jul 10.
Quorum sensing is a system of stimuli and response correlated to population density and involves in pathogen infection, colonization, and pathogenesis. Quorum quenching enzymes as quorum sensing inhibitors have been identified in a number of bacteria and been used to control by triggering the pathogenic phenotype. The marine bacteria of Pseudoalteromonas had wide activity of degrading AHLs as a type of signal molecule associated with quorum sensing. We screened many Pseudoalteromonas strains in large scale to explore genes of quorum quenching enzymes from the China seas by whole-genome sequencing rather than genomic library construction. Nine target strains were obtained and an acylases gene APTM01 from the strain MQS005 belonging to PvdQ type on sub-branch in phylogenetic tree. And the heterogenous host containing the vector with target gene could degrade C10-HSL, C12-HSL and OC12-HSL. The obtained AHL acylase gene would be a candidate quorum quenching gene to apply in some fields. We identified that the strains of Pseudoalteromonas have wide AHL-degrading ability depending on quorum quenching. The strains would be a resource to explore new quorum quenching enzymes.
群体感应是一种与种群密度相关的刺激和反应系统，涉及病原体感染、定植和发病机制。群体感应抑制剂——群体感应淬灭酶已在许多细菌中被发现，并被用于通过触发致病表型来控制。假交替单胞菌的海洋细菌具有广泛的 AHLs 降解活性，AHLs 是一种与群体感应相关的信号分子。我们通过全基因组测序而不是基因组文库构建，从中国海域的大量假交替单胞菌菌株中筛选出能够降解 AHLs 的基因，从而探索海洋细菌的群体感应淬灭酶。得到了 9 株目标菌株，并在系统发育树上属于 PvdQ 亚分支的菌株 MQS005 中发现了酰基转移酶基因 APTM01。含有目标基因的异源宿主可以降解 C10-HSL、C12-HSL 和 OC12-HSL。获得的 AHL 酰基水解酶基因将是一种可应用于某些领域的候选群体感应淬灭基因。我们确定了假交替单胞菌具有广泛的基于群体感应的 AHL 降解能力。这些菌株将成为探索新的群体感应淬灭酶的资源。
