Chen Yue, Shen Qi, Lyu Ping, Lin Renan, Sun Chongbo
Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, People's Republic of China.
Key laboratory of creative Agriculture, Ministry of Agriculture, Hangzhou, People's Republic of China.
Genetica. 2019 Aug;147(3-4):303-313. doi: 10.1007/s10709-019-00071-5. Epub 2019 Jul 10.
Dendrobium officinale, a herb with highly medicinal and ornamental value, is widely distributed in China. MADS-box genes encode transcription factors that regulate various growth and developmental processes in plants, particular in flowering. However, the MADS-box genes in D. officinale are largely unknown. In our study, expression profiling analyses of selected MADS-box genes in D. officinale were performed. In total, 16 DnMADS-box genes with full-length ORF were identified and named according to their phylogenetic relationships with model plants. The transient expression of eight selected MADS-box genes in the epidermal cells of tobacco leaves showed that these DnMADS-box proteins localized to the nucleus. Tissue-specific expression analysis pointed out eight flower-specific expressed MADS-box genes in D. officinale. Furthermore, expression patterns of DnMADS-box genes were investigated during the floral transition process. DnMADS3, DnMADS8 and DnMADS22 were significantly up-regulated in the reproductive phase compared with the vegetative phase, suggesting putative roles of these DnMADS-box genes in flowering. Our data showed that the expressions of MADS-box genes in D. officinale were controlled by diverse exogenous phytohormones. Together, these findings will facilitate further studies of MADS-box genes in Orchids and broaden our understanding of the genetics of flowering.
铁皮石斛是一种具有很高药用和观赏价值的草本植物，在中国广泛分布。MADS-box基因编码转录因子，调控植物的各种生长发育过程，尤其是开花过程。然而，铁皮石斛中的MADS-box基因在很大程度上尚不明确。在我们的研究中，对铁皮石斛中选定的MADS-box基因进行了表达谱分析。总共鉴定出16个具有全长开放阅读框的DnMADS-box基因，并根据它们与模式植物的系统发育关系进行命名。在烟草叶片表皮细胞中对8个选定的MADS-box基因进行瞬时表达分析，结果表明这些DnMADS-box蛋白定位于细胞核。组织特异性表达分析指出铁皮石斛中有8个花特异性表达的MADS-box基因。此外，还研究了DnMADS-box基因在花期转变过程中的表达模式。与营养期相比，DnMADS3、DnMADS8和DnMADS22在生殖期显著上调，表明这些DnMADS-box基因在开花过程中可能发挥作用。我们的数据表明，铁皮石斛中MADS-box基因的表达受多种外源植物激素的调控。总之，这些发现将有助于进一步研究兰花中的MADS-box基因，并拓宽我们对开花遗传学的理解。
