College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, China.
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, 310036, China.
Sci Rep. 2017 Mar 15;7(1):187. doi: 10.1038/s41598-017-00292-8.
Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. officinale. In this study, the contents of polysaccharides and alkaloids in various organs of D. officinale were measured and compared. We took advantage of transcriptomes from four organs to explore biological mechanisms in the organ-specific distribution of active ingredients in D. officinale. Based on Kyoto Encyclopedia of Genes and Genomes pathways, unigenes related to the enzymes involved in fructose and mannose metabolism and unigenes associated with putative upstream elements of the alkaloid biosynthetic pathway were identified. A large number of candidates, including 35 full-length glycosyltransferase genes and 49 full-length P450 genes, were also identified based on the transcriptome data, and the organ-specific expression pattern of these genes was determined. Furthermore, differential expression of all candidate genes was analyzed in two Dendrobium species, D. nobile L. and D. officinale. The data will supply important clues to exploit useful genes involved in polysaccharide and alkaloid synthesis.
铁皮石斛是中国具有高商业价值的重要传统草本植物。据报道，几种生物活性成分，包括多糖和生物碱，对铁皮石斛的优异药用效果做出了重大贡献。在本研究中，测量并比较了铁皮石斛不同器官中的多糖和生物碱含量。我们利用来自四个器官的转录组来探索铁皮石斛中活性成分在器官特异性分布的生物学机制。基于京都基因与基因组百科全书途径，鉴定了与果糖和甘露糖代谢相关酶的基因和与生物碱生物合成途径的假定上游元件相关的基因。还根据转录组数据鉴定了大量的候选基因，包括 35 个全长糖基转移酶基因和 49 个全长 P450 基因，并确定了这些基因的器官特异性表达模式。此外，还分析了两个铁皮石斛物种铁皮石斛和铁皮石斛中所有候选基因的差异表达。这些数据将为开发参与多糖和生物碱合成的有用基因提供重要线索。
