Laboratório de Mutagênese, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Laboratório de Virologia Animal, Instituto de Patologia Tropical, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Environ Sci Pollut Res Int. 2019 Sep;26(26):26553-26562. doi: 10.1007/s11356-019-05882-9. Epub 2019 Jul 10.
Our study evaluated 163 individuals, being 74 soybean farmers, occupationally exposed to pesticides, and 89 individuals from Goias municipalities, Central Brazil, with similar conditions to the exposed group, comprising the control group. Of the 74 soybean farmers, 43 exposed directly to pesticides and 31 exposed indirectly. The exposed group consisted of individuals aged 19 to 63 years, 21 women and 53 men, and the control group had ages ranging from 18 to 64 years, being 36 women and 53 men. 18.9% of the exposed group were poisoned by pesticides, and the most common symptoms were headache and gastrointestinal problems. The genotype frequencies of the rs2031920 (T>C) polymorphism in the CYP2E1 gene present significant differences between the exposed and control groups (p = 0.02), showing that 24.3% of the exposed group were heterozygotes against 6.7% in the control group. For the OGG1 gene, two SNPs, rs1052133 (G>C) and rs293795 (T>C), were evaluated and the genotype frequencies were not statistically different between the exposed and control groups. The DNA damage was distinct (p < 0.05) in the three analyzed comet parameters (tail length, Olive tail moment, %DNA) between groups. However, there was no influence of age and alcohol consumption between the groups associated with the polymorphisms in the CYP2E1 and OGG1 genes and DNA damage. We also did not find altered hematological and biochemical parameters in the exposed group. Thus, this pioneering study at Goias State carried out an overview of the health of soybean farmers. We evaluated classic laboratory exams, associated with exposure markers (comet assay) and susceptibility markers (genetic polymorphisms), emphasizing the need to expand the Brazilian health assessment protocol. We found, in soybean farmers, increased DNA damage and a higher number of heterozygotes in CYP2E1 gene, compared with the control group, despite the lack of association with age, educational level, smoking, drinking habits, and genetic polymorphisms.
我们的研究评估了 163 个人，其中 74 人为职业性接触农药的大豆种植户，89 人来自巴西中部戈亚斯州的与暴露组具有相似条件的市镇，作为对照组。在 74 名大豆种植户中，43 人直接接触农药，31 人间接接触。暴露组由年龄在 19 至 63 岁之间的 21 名女性和 53 名男性组成，对照组的年龄范围为 18 至 64 岁，包括 36 名女性和 53 名男性。暴露组中有 18.9%的人被农药中毒，最常见的症状是头痛和胃肠道问题。CYP2E1 基因 rs2031920（T>C）多态性的基因型频率在暴露组和对照组之间存在显著差异（p=0.02），表明暴露组中有 24.3%的人为杂合子，而对照组中为 6.7%。对于 OGG1 基因，评估了两个 SNPs，rs1052133（G>C）和 rs293795（T>C），暴露组和对照组之间的基因型频率没有统计学差异。在三个分析的彗星参数（尾部长度、橄榄尾部矩、%DNA）中，两组之间的 DNA 损伤明显不同（p<0.05）。然而，在 CYP2E1 和 OGG1 基因和 DNA 损伤的多态性与年龄和饮酒之间没有影响。我们也没有发现暴露组的血液学和生化参数发生改变。因此，这项在戈亚斯州进行的开创性研究对大豆种植户的健康状况进行了概述。我们评估了经典的实验室检查，以及与暴露标志物（彗星试验）和易感性标志物（遗传多态性）相关的检查，强调需要扩大巴西的健康评估方案。我们发现，与对照组相比，大豆种植户的 DNA 损伤增加，CYP2E1 基因的杂合子数量更多，尽管与年龄、教育程度、吸烟、饮酒习惯和遗传多态性无关。
