Ongrádi Joseph, Chatlynne Louise G, Tarcsai Katalin Réka, Stercz Balázs, Lakatos Béla, Pring-Åkerblom Patricia, Gooss Donald, Nagy Károly, Ablashi Dharam V
Department of Medical Microbiology, Semmelweis University, Budapest, Hungary.
National Institute of Dermato-Venereology, Budapest, Hungary.
Front Microbiol. 2019 Jun 25;10:1430. doi: 10.3389/fmicb.2019.01430. eCollection 2019.
An adenovirus (AdV) has been isolated from the rectal swab of a domestic cat () and named feline adenovirus (FeAdV) isolate. It replicates and causes cytopathological effects in many human, feline, other mammalian cell lines that have both Coxsackie-adenovirus-receptor and integrins. Its antigens cross-react with anti-human adenovirus antibodies in immunofluorescence and immunocytochemistry assays. Electron microscopy revealed typical extracellular icosahedral particles and pseudo arrays inside cells. Sequence analysis of hexon and fiber genes indicates that this virus might belong to human adenovirus (HAdV) C species and might be a variant of type 1. In the fiber protein, three altered amino acids occur in the shaft; four altered residues are found in the knob region as compared to a European HAdV might be type 1 isolate (strain 1038, D11). One alteration affects amino acid 442 forming an RGS motif in an alanine rich region that might be an alternative way to bind integrins with subsequent internalization. Substitutions in the hexon sequence are silent. As compared to published HAdV sequences, the fiber is related to the original American prototype and recently described Taiwanese HAdV 1 isolates, but the hexon sequences are related to adenovirus isolates from France, Germany, Japan, and Taiwan. Serology carried out on FeAdV infected M426 cells indicates a prevalence of IgG in 80% of domestic cats in Delaware, United States. FeAdV isolate seems to be a recently recognized virus with possible pathogenic effects and, simultaneous human and feline infections are possible. Further molecular and biological characterization of this feline adenovirus isolate, as well as studies on both human and feline epidemiology and pathomechanisms, especially in endangered big cats, are warranted. FeAdV might have further practical advantages. Namely, it could be utilized in both human and feline AIDS research, developed into diagnostic tools, and gene therapy vectors in the near future.
从一只家猫的直肠拭子中分离出一种腺病毒（AdV），命名为猫腺病毒（FeAdV）分离株。它能在许多同时具有柯萨奇病毒 - 腺病毒受体和整合素的人、猫及其他哺乳动物细胞系中复制并引起细胞病变效应。在免疫荧光和免疫细胞化学检测中，其抗原与抗人腺病毒抗体发生交叉反应。电子显微镜显示细胞外有典型的二十面体颗粒以及细胞内的假晶格。六邻体和纤维基因的序列分析表明，该病毒可能属于人腺病毒（HAdV）C种，可能是1型的变体。在纤维蛋白中，杆部出现三个氨基酸改变；与欧洲HAdV 1分离株（菌株1038，D11）相比，在球状区域发现四个氨基酸残基改变。其中一个改变影响第442位氨基酸，在富含丙氨酸的区域形成一个RGS基序，这可能是一种与整合素结合并随后内化的替代方式。六邻体序列中的替换是沉默的。与已发表的HAdV序列相比，纤维与原始美国原型以及最近描述的台湾HAdV 1分离株相关，但六邻体序列与来自法国、德国、日本和台湾的腺病毒分离株相关。对感染FeAdV的M426细胞进行的血清学检测表明，在美国特拉华州80%的家猫中存在IgG。FeAdV分离株似乎是一种最近才被认识的病毒，可能具有致病作用，人和猫可能会同时感染。有必要对这种猫腺病毒分离株进行进一步的分子和生物学特性研究，以及对人和猫的流行病学及发病机制进行研究，特别是在濒危大型猫科动物中。FeAdV可能还有其他实际优势。也就是说，它可用于人和猫的艾滋病研究，在不久的将来开发成诊断工具和基因治疗载体。
