Pedullà Eugenio, Genovese Carlo, Messina Riccardo, La Rosa Giusy R M, Corsentino Giacomo, Rapisarda Silvia, Arias-Moliz Maria T, Tempera Gianna, Grandini Simone
Department of General Surgery and Medical - Surgical Specialties, University of Catania, Italy.
Department of Biomedical Sciences, University of Catania, Italy.
J Investig Clin Dent. 2019 Nov;10(4):e12434. doi: 10.1111/jicd.12434. Epub 2019 Jul 11.
The aim of the present study was to evaluate the immediate and residual antimicrobial activity of cordless sonic or ultrasonic devices on Enterococcus faecalis (E faecalis)-infected teeth.
A total of 140 single-rooted extracted teeth with E faecalis were grouped as follows (N = 15): conventional syringe irrigation with 3% sodium hypochlorite activated by ultrasonic device (group 1) or cordless ultrasonic device (group 2), EndoActivator (group 3) or without activation (group 4), conventional syringe irrigation with sterile bi-distilled water and ultrasonic device (group 5), EndoUltra (group 6), EndoActivator (group 7), or without activation (group 8). The remaining 20 teeth were used for positive and negative controls. Colony-forming units (CFU) and turbidity were recorded from bacteriological samples taken before and after irrigation and after 24 hours of re-incubation. Data were statistically analyzed using Student's t test, Mann-Whitney test, Kruskal-Wallis test, and Dunn's multiple comparison tests (P < .05).
Groups 1 and 2 showed higher antimicrobial efficacy than groups 3 and 4 (P < .05). No bacteria reduction was found in groups 5-8 (P > .05). After 24 hours, regrowth of bacteria was not significantly different for all groups (P > .05).
Ultrasound produced lower CFU and turbidity after treatment and after re-incubation of 24 hours than sonic or no activation.
本研究旨在评估无绳声波或超声波设备对粪肠球菌感染牙齿的即时和残留抗菌活性。
将140颗感染粪肠球菌的单根离体牙分为以下几组（每组n = 15）：用超声波设备激活的3%次氯酸钠通过传统注射器冲洗（第1组）或无绳超声波设备（第2组），EndoActivator（第3组）或不激活（第4组），用无菌双蒸水和超声波设备进行传统注射器冲洗（第5组），EndoUltra（第6组），EndoActivator（第7组），或不激活（第8组）。其余20颗牙齿用作阳性和阴性对照。记录冲洗前后以及再培养24小时后细菌学样本中的菌落形成单位（CFU）和浊度。使用学生t检验、曼-惠特尼检验、克鲁斯卡尔-沃利斯检验和邓恩多重比较检验对数据进行统计学分析（P < 0.05）。
第1组和第2组的抗菌效果高于第3组和第4组（P < 0.05）。第5 - 8组未发现细菌减少（P > 0.05）。24小时后，所有组的细菌再生长无显著差异（P > 0.05）。
与声波或无激活相比，超声治疗后以及再培养24小时后产生的CFU和浊度更低。
