Department of Conservative Dentistry, King's College London Dental Institute, London, UK.
Int Endod J. 2010 Mar;43(3):241-50. doi: 10.1111/j.1365-2591.2009.01672.x.
To compare the efficacy of passive ultrasonic irrigation with 1% sodium hypochlorite, with that of conventional syringe irrigation with 1% sodium hypochlorite, on intraradicular Enterococcus faecalis biofilms in extracted single-rooted human teeth.
Biofilms of E. faecalis (strain OMGS 3202) were grown on the prepared root canal walls of 48 standardized root halves which had been longitudinally sectioned. Following reapproximation, the roots were divided into four groups of twelve. The two experimental groups were treated with conventional syringe irrigation with 1% sodium hypochlorite solution (experimental group A) and passive ultrasonic irrigation with 1% sodium hypochlorite solution (experimental group B). Of the two control groups, the first was treated with conventional syringe irrigation with sterile saline solution (control group C), whilst the second control group (D) received no irrigation. The root halves were processed for scanning electron microscopy. Three images (x 700), coronal, middle and apical, were taken of the twelve root halves in each of the four groups, using a standardized protocol. The images were randomized and biofilm coverage assessed independently by three calibrated examiners, using a four-point scoring system.
There were no significant differences in the scores for remaining biofilm coverage between group A (conventional syringe irrigation with 1% sodium hypochlorite) and group B (passive ultrasonic irrigation with 1% sodium hypochlorite) at the three observed levels. There was a significant difference between both experimental groups (groups A and B) and group C (conventional syringe irrigation with sterile saline solution) (P < 0.001) at all three observed levels.
Both conventional syringe irrigation and passive ultrasonic irrigation with 1% sodium hypochlorite were effective at completely removing intraradicular E. faecalis biofilms. Conventional syringe irrigation with sterile saline solution was only partially effective at removing the biofilms.
比较 1%次氯酸钠被动超声冲洗与传统注射器冲洗对离体人单根牙根内粪肠球菌生物膜的疗效。
在 48 个标准化根管壁制备物上培养粪肠球菌（OMGS 3202 株）生物膜。重新接近后，将根分为四组，每组 12 个。实验组 A 用 1%次氯酸钠溶液进行传统注射器冲洗，实验组 B 用 1%次氯酸钠溶液进行被动超声冲洗。对照组 C 用无菌生理盐水进行传统注射器冲洗，对照组 D 不冲洗。将根管半切进行扫描电镜检查。使用标准化方案，对每组的 12 个根管半切取 3 张图像（x700），分别为冠部、中部和根尖部。将图像随机化，由三位经过校准的检查者使用四分制评分系统独立评估生物膜覆盖程度。
在三个观察水平上，组 A（用 1%次氯酸钠溶液进行传统注射器冲洗）和组 B（用 1%次氯酸钠溶液进行被动超声冲洗）之间的剩余生物膜覆盖率评分没有显著差异。在三个观察水平上，实验组 A 和 B 与对照组 C（用无菌生理盐水进行传统注射器冲洗）之间均有显著差异（P <0.001）。
1%次氯酸钠的传统注射器冲洗和被动超声冲洗均能有效彻底清除根管内粪肠球菌生物膜。用无菌生理盐水进行传统注射器冲洗只能部分去除生物膜。
