Göke R, Göke B, Steinfelder H J, Arnold R
Institute of Pharmacology and Toxicology, University of Göttingen, F.R.G.
Int J Pancreatol. 1988 Mar;3(2-3):135-42. doi: 10.1007/BF02798924.
The effects of the low molecular weight serine proteinase inhibitor FOY (gabexate mesilate) upon insulin action was studied in three different experimental systems. Placenta membranes containing insulin receptors preincubated with FOY (10 microM) showed a reduction of insulin-stimulated tyrosine kinase activity (p less than 0.01). However, FOY (0.1-100 microM) did not affect the insulin-stimulated tyrosine kinase activity in a preparation of solubilized and partially purified insulin receptors from placental membranes. Isolated adipocytes were used to study the effect of FOY on intact cells. FOY neither altered the insulin induced inhibition of the catecholamine-stimulated lipolysis nor (at the low concentration of 0.1 mM) the stimulation of glucose transport by the hormone. High concentrations (0.5 mM) of FOY decreased the effect of insulin on the hexose transport.
在三种不同的实验系统中研究了低分子量丝氨酸蛋白酶抑制剂FOY（甲磺酸加贝酯）对胰岛素作用的影响。预先用FOY（10微摩尔）孵育的含有胰岛素受体的胎盘膜显示胰岛素刺激的酪氨酸激酶活性降低（p＜0.01）。然而，FOY（0.1 - 100微摩尔）对从胎盘膜中溶解并部分纯化的胰岛素受体制剂中胰岛素刺激的酪氨酸激酶活性没有影响。使用分离的脂肪细胞来研究FOY对完整细胞的作用。FOY既没有改变胰岛素诱导的对儿茶酚胺刺激的脂解作用的抑制，也没有（在0.1毫摩尔的低浓度下）改变该激素对葡萄糖转运的刺激作用。高浓度（0.5毫摩尔）的FOY降低了胰岛素对己糖转运的作用。
