Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Clin Chim Acta. 2019 Oct;497:41-47. doi: 10.1016/j.cca.2019.07.009. Epub 2019 Jul 8.
A disposable package of biosensors was developed along with the corresponding guidelines for early detection of the acute lymphoblastic leukemia cancer. This proposed cancer-screening package included a DNA sensor and an aptasensor as two main types of biosensors. The biosensors were used simultaneously. This combination of sensors can detect not only the presence of mutant genes but also the biomarkers of cancer. At current work, the combination of sensors were used to detect the presence of BCR-ABL1 as a mutant gene and CEA as a biomarkers of cancer, such a capability makes the package liable for early and certain detection of acute lymphoblastic leukemia. To construct both the DNA sensor and the aptasensor, a nanocomposite consisting of electrosynthesis carbon quantum dots and biosynthesized gold nanoparticles was applied. The construction of these biosensors was characterized using four different electrochemical methods including DPV (Differential Pulse Voltammetry), EIS (Electrochemical Impedance Spectroscopy), CV (Cyclic Voltammetry) and chronoamperometry. The peak current of a catechol solution that was used as an electroactive probe on the biosensor was linearly related to the logarithm of the concentrations of the target DNA and the target antigen in the range of 10 pM to 100 μM and 1 pg mL to 0.001 g mL with the detection limits of 1.5 pM and 0.26 pg mL respectively, which are quite good results.
一种一次性的生物传感器试剂盒与早期检测急性淋巴细胞白血病癌症的相应指南一起开发。该癌症筛查试剂盒包括 DNA 传感器和适体传感器作为两种主要类型的生物传感器。生物传感器同时使用。这种传感器组合不仅可以检测突变基因的存在，还可以检测癌症的生物标志物。在目前的工作中，传感器组合用于检测 BCR-ABL1 作为突变基因和 CEA 作为癌症生物标志物的存在，这种能力使得该试剂盒能够早期且可靠地检测急性淋巴细胞白血病。为了构建 DNA 传感器和适体传感器，应用了由电合成碳量子点和生物合成金纳米粒子组成的纳米复合材料。使用四种不同的电化学方法（包括差分脉冲伏安法（DPV）、电化学阻抗谱法（EIS）、循环伏安法（CV）和计时安培法）对这些生物传感器的构建进行了表征。将儿茶酚溶液作为生物传感器上的电活性探针的峰电流与目标 DNA 和目标抗原在 10 pM 至 100 μM 和 1 pg mL 至 0.001 g mL 的浓度的对数呈线性相关，检测限分别为 1.5 pM 和 0.26 pg mL，这是相当不错的结果。
