Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
Bioelectrochemistry. 2018 Jun;121:38-45. doi: 10.1016/j.bioelechem.2017.12.010. Epub 2018 Jan 16.
In this work we report the synthesis of a stable composite with excellent electrical properties, on the surface of a biosensor. Conductive polymers offer both high electrical conductivity and mechanical strength. Many reports have focused on synthesizing conductive polymers with the aid of high-cost enzymes. In the current work we introduce a novel electrochemical, one-step, facile and cost effective procedure for synthesizing poly (catechol), without using expensive enzymes. The poly (catechol) conductivity was enhanced by modification with graphene sheets and biosynthesized gold nanoparticles. Four different robust methods, DPV, EIS, CV and chronoamperometry, were used to monitor the biosensor modifications. The peak currents of the catechol (an electroactive probe) were linearly related to the logarithm of the concentrations of target DNA in the range 100.0 μM to 10.0 pM, with a detection limit of 1.0 pM for the DNA strand. The current work investigates a new, stable composite consisting of conductive polymers and nanoparticles, which was applied to the detection of acute lymphoblastic leukemia.
在这项工作中，我们报告了一种在生物传感器表面具有优异电性能的稳定复合材料的合成。导电聚合物具有高导电性和机械强度。许多报告都集中在借助昂贵的酶合成导电聚合物上。在目前的工作中，我们提出了一种新颖的电化学、一步法、简便且经济有效的方法，用于合成聚（儿茶酚），而无需使用昂贵的酶。通过用石墨烯片和生物合成的金纳米粒子进行修饰，提高了聚（儿茶酚）的电导率。使用四种不同的稳健方法（DPV、EIS、CV 和计时安培法）监测生物传感器的修饰。儿茶酚（一种电活性探针）的峰电流与目标 DNA 浓度的对数在 100.0 μM 至 10.0 pM 范围内呈线性关系，DNA 链的检测限为 1.0 pM。目前的工作研究了一种由导电聚合物和纳米粒子组成的新型稳定复合材料，该复合材料已应用于急性淋巴细胞白血病的检测。
