Anaya-Esparza Luis M, González-Silva Napoleón, Yahia Elhadi M, González-Vargas O A, Montalvo-González Efigenia, Pérez-Larios Alejandro
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico 255 Fracc, Lagos del Country, Tepic 63175, Nayarit, Mexico.
División de Ciencias Agropecuarias e Ingenierías, Centro Universitario de los Altos, Universidad de Guadalajara, Av. Rafael Casillas Aceves 1200, Tepatitlán de Morelos 47600, Jalisco, Mexico.
Nanomaterials (Basel). 2019 Jul 10;9(7):992. doi: 10.3390/nano9070992.
Mixed oxide nanoparticles (MONs, TiO-ZnO-MgO) obtained by the sol-gel method were characterized by transmission electron microscopy, (TEM, HRTEM, and SAED) and thermogravimetric analysis (TGA/DTGA-DTA). Furthermore, the effect of MONs on microbial growth (growth profiling curve, lethal and sublethal effect) of , , and , as well as the toxicity against by the lethal concentration test (LC) were evaluated. MONs exhibited a near-spherical in shape, polycrystalline structure and mean sizes from 17 to 23 nm. The thermal analysis revealed that the anatase phase of MONs is completed around 480-500 °C. The normal growth of all bacteria tested is affected by the MONs presence compared with the control group. MONs also exhibited a reduction on the plate count from 0.58 to 2.10 log CFU/mL with a sublethal cell injury from 17 to 98%. No significant toxicity within 24 h was observed on . A bacteriostatic effect of MONs on bacteria was evidenced, which was strongly influenced by the type of bacteria, as well as no toxic effects (LC >1000 mg/L; TiO-ZnO (5%)-MgO (5%)) on were detected. This study demonstrates the potential of MONs for industrial applications.
通过溶胶-凝胶法制备的混合氧化物纳米颗粒（MONs，TiO-ZnO-MgO）采用透射电子显微镜（TEM、HRTEM和SAED）和热重分析（TGA/DTGA-DTA）进行了表征。此外，还评估了MONs对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和铜绿假单胞菌的微生物生长（生长曲线、致死和亚致死效应）的影响，以及通过致死浓度试验（LC）对卤虫的毒性。MONs呈近球形，具有多晶结构，平均尺寸为17至23纳米。热分析表明，MONs的锐钛矿相在480-500℃左右完成。与对照组相比，所有受试细菌的正常生长均受到MONs存在的影响。MONs还使平板计数从0.58降至2.10 log CFU/mL，亚致死细胞损伤率为17%至98%。在24小时内未观察到对卤虫有明显毒性。证实了MONs对细菌具有抑菌作用，这受到细菌类型的强烈影响，并且未检测到对卤虫的毒性作用（LC>1000 mg/L；TiO-ZnO（5%）-MgO（5%））。本研究证明了MONs在工业应用中的潜力。
