Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, Brazil.
Laboratório de Plantas Medicinais e Derivados (PN1), Farmanguinhos, FIOCRUZ, Rio de Janeiro 21041-250, Brazil.
Molecules. 2019 Jul 10;24(14):2525. doi: 10.3390/molecules24142525.
The difficulties encountered and the numerous side effects present in the treatment of cutaneous leishmaniasis have encouraged the research for new compounds that can complement or replace existing treatment. The growing scientific interest in the study of plants, which are already used in folk remedies, has led our group to test essential oil against . Several species of the family, or their compounds, have relevant antiprotozoal activities Therefore, the biological potential on forms from the essential oil of leaves was verified for the first time in that work. The antileishmanial activity was evaluated against promastigotes and intracellular amastigotes, and cytotoxicity were performed with J774.G8, which were incubated with different concentrations of essential oil. Transmission electron microscopy and flow cytometry were performed with essential oil IC. Promastigote forms showed essential oil IC of 7.945 ± 1.285 µg/mL (24 h) and 6.186 ± 1.226 µg/mL (48 h), while for intracellular amastigote forms it was 3.546 ± 1.184 µg/mL (24 h). The CC was 15.14 ± 0.090 µg/mL showing that essential oil is less toxic to macrophages than to parasites. Transmission electron microscopy showed that essential oil treatment is capable of inducing mitochondrial damage to promastigote and intracellular amastigote forms, while flow cytometry showed ΔѰm disruption in treated parasites. These results could bring about new possibilities to develop products based on essential oil to treat cutaneous leishmaniasis, especially for people who cannot receive the conventional therapy.
在治疗皮肤利什曼病时遇到的困难和众多的副作用促使人们研究新的化合物，可以补充或替代现有的治疗方法。对植物的科学研究兴趣日益浓厚，这些植物已经在民间疗法中使用，这促使我们的研究小组对 进行了精油测试。 科的几种物种，或它们的化合物，具有相关的抗原生动物活性。因此，首次在该工作中验证了 叶精油对 形式的生物学潜力。抗利什曼原虫活性针对前鞭毛体和细胞内无鞭毛体进行评估，并使用 J774.G8 进行细胞毒性测试，该细胞与不同浓度的 精油孵育。用 精油 IC 进行了透射电子显微镜和流式细胞术检查。前鞭毛体形式的 精油 IC 为 7.945 ± 1.285 µg/mL（24 h）和 6.186 ± 1.226 µg/mL（48 h），而细胞内无鞭毛体形式的 IC 为 3.546 ± 1.184 µg/mL（24 h）。CC 为 15.14 ± 0.090 µg/mL，表明 精油对巨噬细胞的毒性比寄生虫低。透射电子显微镜显示， 精油处理能够诱导前鞭毛体和细胞内无鞭毛体形式的线粒体损伤，而流式细胞术显示处理后的寄生虫中 ΔѰm 破坏。这些结果可能为开发基于 精油的产品治疗皮肤利什曼病带来新的可能性，特别是对于不能接受常规治疗的人。
