School of Biochemical Engineering, IIT (BHU), Varanasi, 221005, India.
Centre of Bioinformatics, University of Allahabad, Prayagraj, 211002, India.
Biometals. 2023 Aug;36(4):829-845. doi: 10.1007/s10534-022-00476-4. Epub 2022 Dec 1.
In the present work, the removal of Cr (VI), Cd (II) and Pb (II) at 50 mg/L of each metal ion concentration was investigated by Microbacterium paraoxydans strain VSVM IIT(BHU). The heavy metal binding on the bacterial cell surface was confirmed through X-ray photoelectron spectroscopy and energy dispersive X-ray. X-ray photoelectron spectroscopy analysis also confirmed the reduction of Cr (VI) to Cr (III). Heavy metal removal dynamics was investigated by evaluating dimensionless, and the value of N (9.49 × 10, 9.92 × 10 and 1.23 × 10 for Cr (VI), Cd (II) and Pb (II) ions) indicated that the removal of heavy metals by bacterial isolate was mixed diffusion and transfer controlled. It was found that both the experimental and predicted values for isolated bacterial strain coincided with each other with a good R value in the L-M Algorithm range of 0.94-0.98 for the ternary metal ion system. The bacterial isolate presented a maximum heavy metal ion removal efficiency of 91.62% Cr (VI), 89.29% Pb (II), and 83.29% Cd (II) at 50 mg/L.
在本工作中，通过微杆菌属 VSVM IIT(BHU) 菌株研究了 50mg/L 每种金属离子浓度下 Cr(VI)、Cd(II) 和 Pb(II) 的去除情况。通过 X 射线光电子能谱和能量色散 X 射线证实了重金属在细菌细胞表面的结合。X 射线光电子能谱分析还证实了 Cr(VI)被还原为 Cr(III)。通过评估无因次数 N（9.49×10、9.92×10 和 1.23×10 分别对应 Cr(VI)、Cd(II)和 Pb(II)离子）来研究重金属去除动力学，表明细菌分离物对重金属的去除是混合扩散和传递控制的。发现对于三元金属离子体系，L-M 算法范围内的 0.94-0.98 的良好 R 值，实验值和预测值与分离出的细菌菌株的对应值相吻合。细菌分离物在 50mg/L 时对重金属离子的去除效率最高，Cr(VI)为 91.62%，Pb(II)为 89.29%，Cd(II)为 83.29%。
