使用集成光声显微镜和光学相干断层扫描技术进行视网膜和脉络膜成像。
Retinal and choroidal imaging using integrated photoacoustic microscopy and optical coherence tomography.
作者信息
Tian Chao, Zhang Wei, Nguyen Van Phuc, Huang Ziyi, Wang Xueding, Paulus Yannis M
机构信息
Kellogg Eye Center, Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48105, USA.
出版信息
Proc SPIE Int Soc Opt Eng. 2018 Jan-Feb;10474. doi: 10.1117/12.2290667. Epub 2018 Feb 19.
Most reported photoacoustic ocular imaging work to date uses small animals, such as mice and rats, the eyes of which are small and less than one-third the size of a human eye, which poses a challenge for clinical translation. Here we achieved chorioretinal imaging of larger animals, i.e. rabbits, using a dual-modality photoacoustic microscopy (PAM) and optical coherence tomography (OCT) system. Preliminary experimental results in living rabbits demonstrate that the PAM can noninvasively visualize depth-resolved retinal and choroidal vessels using a safe laser exposure dose; and the OCT can finely distinguish different retinal layers, the choroid, and the sclera. This reported work might be a major step forward in clinical translation of photoacoustic microscopy.
迄今为止，大多数已报道的光声眼部成像研究使用的是小动物，如小鼠和大鼠，它们的眼睛很小，不到人眼大小的三分之一，这给临床转化带来了挑战。在此，我们使用双模态光声显微镜（PAM）和光学相干断层扫描（OCT）系统，实现了对较大动物（即兔子）的脉络膜视网膜成像。对活体兔子的初步实验结果表明，PAM能够使用安全的激光照射剂量，无创地可视化深度分辨的视网膜和脉络膜血管；而OCT能够精细地区分不同的视网膜层、脉络膜和巩膜。这项已报道的工作可能是光声显微镜临床转化中的一个重要进展。
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