Tian Chao, Zhang Wei, Nguyen Van Phuc, Wang Xueding, Paulus Yannis M
Kellogg Eye Center, Department of Ophthalmology and Visual Sciences, University of Michigan.
Department of Biomedical Engineering, University of Michigan; Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College.
J Vis Exp. 2018 Feb 8(132):57135. doi: 10.3791/57135.
Photoacoustic ocular imaging is an emerging ophthalmic imaging technology that can noninvasively visualize ocular tissue by converting light energy into sound waves and is currently under intensive investigation. However, most reported work to date is focused on the imaging of the posterior segment of the eyes of small animals, such as rats and mice, which poses challenges for clinical human translation due to small eyeball sizes. This manuscript describes a novel photoacoustic microscopy (PAM) and optical coherence tomography (OCT) dual-modality system for posterior segment imaging of the eyes of larger animals, such as rabbits. The system configuration, system alignment, animal preparation, and dual-modality experimental protocols for in vivo, noninvasive, label-free chorioretinal imaging in rabbits are detailed. The effectiveness of the method is demonstrated through representative experimental results, including retinal and choroidal vasculature obtained by the PAM and OCT. This manuscript provides a practical guide to reproducing the imaging results in rabbits and advancing photoacoustic ocular imaging in larger animals.
光声眼部成像技术是一种新兴的眼科成像技术，它能够通过将光能转化为声波来无创地可视化眼部组织，目前正受到深入研究。然而，迄今为止，大多数已报道的工作都集中在对大鼠和小鼠等小动物的眼后段成像上，由于眼球尺寸较小，这给临床人体翻译带来了挑战。本文描述了一种用于较大动物（如兔子）眼后段成像的新型光声显微镜（PAM）和光学相干断层扫描（OCT）双模态系统。详细介绍了该系统的配置、系统校准、动物准备以及用于兔子体内无创、无标记脉络膜视网膜成像的双模态实验方案。通过代表性的实验结果证明了该方法的有效性，包括通过PAM和OCT获得的视网膜和脉络膜血管系统。本文为在兔子身上重现成像结果以及推进较大动物的光声眼部成像提供了实用指南。
