兔视网膜新生血管的高分辨率、体内多模态光声显微镜、光学相干断层扫描和荧光显微镜成像

High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization.

作者信息

Zhang Wei, Li Yanxiu, Nguyen Van Phuc, Huang Ziyi, Liu Zhipeng, Wang Xueding, Paulus Yannis M

机构信息

1Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48105 USA.

2Institution of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192 China.

出版信息

Light Sci Appl. 2018 Dec 5;7:103. doi: 10.1038/s41377-018-0093-y. eCollection 2018.

Photoacoustic microscopy (PAM) is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes. This report describes an integrated multimodality imaging system that combines PAM, optical coherence tomography (OCT), and fluorescence microscopy (FM) to evaluate angiogenesis in larger animal eyes. High-resolution in vivo imaging was performed in live rabbit eyes with vascular endothelial growth factor (VEGF)-induced retinal neovascularization (RNV). The results demonstrate that our multimodality imaging system can non-invasively visualize RNV in both albino and pigmented rabbits to determine retinal pathology using PAM and OCT and verify the leakage of neovascularization using FM and fluorescein dye. This work presents high-resolution visualization of angiogenesis in rabbits using a multimodality PAM, OCT, and FM system and may represent a major step toward the clinical translation of the technology.

光声显微镜（PAM）是一种新兴的成像技术，可在动物眼中无创地可视化眼部结构。本报告描述了一种集成的多模态成像系统，该系统结合了PAM、光学相干断层扫描（OCT）和荧光显微镜（FM），用于评估大型动物眼中的血管生成。在患有血管内皮生长因子（VEGF）诱导的视网膜新生血管（RNV）的活兔眼中进行了高分辨率体内成像。结果表明，我们的多模态成像系统可以在白化兔和有色兔中无创地可视化RNV，使用PAM和OCT确定视网膜病变，并使用FM和荧光素染料验证新生血管的渗漏。这项工作展示了使用多模态PAM、OCT和FM系统对兔血管生成进行高分辨率可视化，可能代表了该技术向临床转化的重要一步。