Królak M, Błaszczyk B
Pracownia Badania Brucelozy Instytutu Weterynarii w Puławach z siedziba w Gdańsku.
Pol Arch Weter. 1986;25(1):49-61.
A differentiated time and temperature of incubation were used in the first CFT phase. It was found that the activity of complement fixation (C') decreased in the reaction medium. This was observed in the control system in which at 1 C'H50 hemolysis inhibition increase from 50 to 90% was found after 2 hr at 37 degrees C, after 8 hr at 20 degrees C and after 18 hr at 4 degrees C. Simultaneously, a double increase of the standard serum titre was obtained at 4 and 20 degrees C as compared with that at 37 degrees C. At stable concentration of the antigen and complement the effect of C' fixation was the strongest at 4 degrees C, slightly weaker at 20 degrees C and the weakest at 37 degrees C. The above dependence resulted from a different reaction distribution in the chessboard system at each temperature. In the order from the lowest to the highest antigen dilutions positive reactions were increasing and then subsequently expired at 4, 20 and 37 degrees C, forming an optimal antigen dilution typical for each temperature. In our own standard CFT technique a heat and short C' fixation (37 degrees C for 30 min) were chosen which provided the required reaction sensitivity and permitted to make mass diagnostic examinations. It was also shown that the temperature and time of initial incubation of the antigen-antibody complex had no influence on the effect of C' fixation by this complex. This is supported by joint introduction of the antigen and complement into sera.
在补体结合试验（CFT）的第一阶段使用了不同的孵育时间和温度。发现在反应介质中补体结合（C'）活性降低。在控制系统中观察到，在37℃孵育2小时、20℃孵育8小时和4℃孵育18小时后，1个补体单位（C'H50）溶血抑制率从50%增加到90%。同时，与37℃相比，在4℃和20℃时标准血清效价提高了两倍。在抗原和补体浓度稳定的情况下，C'结合效应在4℃时最强，在20℃时稍弱，在37℃时最弱。上述依赖性是由每个温度下棋盘系统中不同的反应分布导致的。从最低到最高抗原稀释度，阳性反应在4℃、20℃和37℃时先增加然后随后消失，形成每个温度特有的最佳抗原稀释度。在我们自己的标准CFT技术中，选择了热和短时间的C'结合（37℃ 30分钟），这提供了所需的反应灵敏度并允许进行大规模诊断检查。还表明抗原 - 抗体复合物的初始孵育温度和时间对该复合物的C'结合效果没有影响。这通过将抗原和补体共同加入血清得到了支持。
