Daltaban Iskender Samet, Misir Sema, Turksoy Vugar Ali, Ak Hakan, Cakir Ertugrul
Department of Neurosurgery, Bozok University Faculty of Medicine, Yozgat, Turkey.
Department of Biochemistry, Cumhuriyet University Faculty of Pharmacy, Sivas, Turkey.
North Clin Istanb. 2018 Oct 9;6(2):103-109. doi: 10.14744/nci.2018.89411. eCollection 2019.
Increased intracellular calcium concentration plays an important role in the secondary mechanism of spinal cord injury. In the presenting experimental study, we aimed to evaluate the healing effect of barnidipine, which has a high affinity for L-type calcium channels, on acute spinal cord injury and to compare its effects with those of methylprednisolone.
A total of 32 Spraque Dawley albino adult female rats were divided into 4 groups; group 1: sham-operated (n=8), group 2: only ischemia (n=6), group 3: barnidipine-treated (n=8), and group 4: methylprednisolone-treated (n=6). An ischemia-reperfusion model was created by clipping the abdominal aorta in the rats. Motor examination was performed 1 hour after the surgical procedure and before sacrification. Immediately following the second motor examination, rats were sacrificed and tissue samples were taken for histopathological examination and for testing of tissue malondialdehyde (MDA) levels.
A significant correlation of motor examination was found between the sham-operated and barnidipine-treated groups and the sham-operated and only ischemia groups at the 1 and 24 hour (p<0.008). There was no significant difference between the only ischemia and barnidipine-treated groups and only ischemia and methylprednisolone-treated groups (p>0.008). Light microscopic examination of the sham-operated group revealed findings consistent with normal spinal cord structure. In group 2, 3, and 4, light microscopic examination revealed polymorphonuclear leukocyte infiltration and a small amount of axonal swelling. There was no significant correlation between the ischemia and barnidipine-treated groups and the barnidipine and methylprednisolone groups in terms of MDA levels (p>0.008).
A single dose of barnidipine (10 mg/kg) and methylprednisolone are not effective and not sufficient to prevent spinal ischemia-reperfusion injury in rats.
细胞内钙浓度升高在脊髓损伤的继发机制中起重要作用。在本实验研究中，我们旨在评估对L型钙通道具有高亲和力的巴尼地平对急性脊髓损伤的愈合效果，并将其效果与甲基强的松龙的效果进行比较。
总共32只成年雌性斯普拉格·道利白化大鼠被分为4组；第1组：假手术组（n = 8），第2组：仅缺血组（n = 6），第3组：巴尼地平治疗组（n = 8），第4组：甲基强的松龙治疗组（n = 6）。通过夹闭大鼠腹主动脉建立缺血再灌注模型。在手术操作后1小时和处死前进行运动检查。在第二次运动检查后立即处死大鼠，并取组织样本进行组织病理学检查和组织丙二醛（MDA）水平检测。
在1小时和24小时时，假手术组与巴尼地平治疗组以及假手术组与仅缺血组之间的运动检查存在显著相关性（p < 0.008）。仅缺血组与巴尼地平治疗组之间以及仅缺血组与甲基强的松龙治疗组之间无显著差异（p > 0.008）。假手术组的光镜检查结果显示与正常脊髓结构一致。在第2、3和4组中，光镜检查显示多形核白细胞浸润和少量轴突肿胀。在MDA水平方面，缺血组与巴尼地平治疗组以及巴尼地平组与甲基强的松龙组之间无显著相关性（p > 0.008）。
单剂量的巴尼地平（10 mg/kg）和甲基强的松龙对预防大鼠脊髓缺血再灌注损伤无效且不足够。
