MiR-506-3p通过靶向GALNT4在前列腺癌中作为一种新型肿瘤抑制因子发挥作用。
MiR-506-3p acts as a novel tumor suppressor in prostate cancer through targeting GALNT4.
作者信息
Hu C-Y, You P, Zhang J, Zhang H, Jiang N
机构信息
Department of Urology, Gongli Hospital Affiliated to The Second Military Medical University, Shanghai, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Jun;23(12):5133-5138. doi: 10.26355/eurrev_201906_18177.
OBJECTIVE
Researches have indicated that microRNA-506-3p (miR-506-3p) was downregulated and functioned as tumor suppressor in cancers. However, the biological role of miR-506-3p in prostate cancer (PCa) remains to be elucidated.
MATERIALS AND METHODS
Expression of miR-506-3p in PCa cell lines was measured by qRT-PCR. Effects of miR-506-3p expression on PCa cell behaviors were investigated with MTT assay, colony formation assay, and transwell invasion assay. Connection of miR-506-3p and N-Acetylgalactosaminyltransferase-4 (GALNT4) was analyzed with luciferase activity reporter assay and Western blot assay.
RESULTS
miR-506-3p expression was downregulated in PCa cell lines. Function studies demonstrated that overexpression of miR-506-3p inhibits PCa tumor progression in vitro. Mechanistic investigations found GALNT4 was a direct target of miR-506-3p. Overexpression of GALNT4 reversed the tumor-suppressive effects of miR-506-3p on PCa cell.
CONCLUSIONS
Our results elucidated genetic silencing of miR-506-3p enhances GALNT4 oncogene expression to accelerate PCa progression.
目的
研究表明，微小RNA-506-3p（miR-506-3p）在癌症中表达下调并发挥肿瘤抑制作用。然而，miR-506-3p在前列腺癌（PCa）中的生物学作用仍有待阐明。
材料与方法
采用qRT-PCR检测miR-506-3p在PCa细胞系中的表达。通过MTT法、集落形成试验和Transwell侵袭试验研究miR-506-3p表达对PCa细胞行为的影响。用荧光素酶活性报告试验和蛋白质印迹试验分析miR-506-3p与N-乙酰半乳糖胺基转移酶-4（GALNT4）的关系。
结果
miR-506-3p在PCa细胞系中表达下调。功能研究表明，miR-506-3p的过表达在体外抑制PCa肿瘤进展。机制研究发现GALNT4是miR-506-3p的直接靶点。GALNT4的过表达逆转了miR-506-3p对PCa细胞的肿瘤抑制作用。
结论
我们的结果表明，miR-506-3p的基因沉默增强了GALNT4癌基因的表达，从而加速了PCa的进展。
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