Urology Department, Peking Union Medical College Hospital, No. 1 ShuaiFuYuan, DongCheng District, Beijing, P. R. China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):539-546. doi: 10.26355/eurrev_201901_16866.
microRNAs (miRNAs) were regarded as critical participators for human cancers progression including prostate cancer (PCa) and have the potential to be used as treatment targets for cancers. Herein, we validated a tumor-suppressive miRNA, miR-490-3p, which may suppress PCa progression. Histone deacetylase 2 (HDAC2) is a protein that aberrantly expressed in several cancers. However, the role of HDAC2 in the progression of PCa has not been fully elucidated.
Expression of miR-490-3p and HDAC2 in PCa was investigated. The effects of miR-490-3p or HDAC2 expression on PCa cell behaviors were analyzed. Association between miR-490-3p and HDAC2 was analyzed by luciferase activity reporter assay and Western blot assay.
We demonstrated that miR-490-3p functioned as a tumor-suppressive role in PCa progression. We found miR-490-3p expression was decreased in PCa cell lines. Down-regulation of miR-490-3p promoted the growth, migration, invasion but inhibited apoptosis of PCa cells. HDAC2 was validated as a direct target of miR-490-3p and promoted the progression of PCa cells. Further studies showed that HDAC2 could reverse the effects of miR-490-3p on growth, migration, invasion and apoptosis of PCa cells.
Our data highlighted the key role of miR-490-3p in the progression of PCa. Thus, miR-490-3p may be a novel cancer-specific therapeutic.
微小 RNA（miRNA）被认为是人类癌症进展的关键参与者，包括前列腺癌（PCa），并有可能成为癌症治疗的靶点。在此，我们验证了一种肿瘤抑制 miRNA，miR-490-3p，它可能抑制 PCa 的进展。组蛋白去乙酰化酶 2（HDAC2）是一种在多种癌症中异常表达的蛋白质。然而，HDAC2 在 PCa 进展中的作用尚未完全阐明。
研究了 miR-490-3p 和 HDAC2 在 PCa 中的表达。分析了 miR-490-3p 或 HDAC2 表达对 PCa 细胞行为的影响。通过荧光素酶活性报告基因检测和 Western blot 检测分析 miR-490-3p 和 HDAC2 之间的关联。
我们证明 miR-490-3p 在 PCa 进展中发挥肿瘤抑制作用。我们发现 miR-490-3p 在 PCa 细胞系中的表达降低。下调 miR-490-3p 促进了 PCa 细胞的生长、迁移、侵袭，但抑制了其凋亡。HDAC2 被验证为 miR-490-3p 的直接靶标，并促进了 PCa 细胞的进展。进一步的研究表明，HDAC2 可以逆转 miR-490-3p 对 PCa 细胞生长、迁移、侵袭和凋亡的影响。
我们的数据强调了 miR-490-3p 在 PCa 进展中的关键作用。因此，miR-490-3p 可能是一种新的癌症特异性治疗方法。
