miR-490-3p 通过调控组蛋白去乙酰化酶 2 来调节前列腺癌的进展。
miR-490-3p modulates the progression of prostate cancer through regulating histone deacetylase 2.
机构信息
Urology Department, Peking Union Medical College Hospital, No. 1 ShuaiFuYuan, DongCheng District, Beijing, P. R. China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):539-546. doi: 10.26355/eurrev_201901_16866.
OBJECTIVE
microRNAs (miRNAs) were regarded as critical participators for human cancers progression including prostate cancer (PCa) and have the potential to be used as treatment targets for cancers. Herein, we validated a tumor-suppressive miRNA, miR-490-3p, which may suppress PCa progression. Histone deacetylase 2 (HDAC2) is a protein that aberrantly expressed in several cancers. However, the role of HDAC2 in the progression of PCa has not been fully elucidated.
MATERIALS AND METHODS
Expression of miR-490-3p and HDAC2 in PCa was investigated. The effects of miR-490-3p or HDAC2 expression on PCa cell behaviors were analyzed. Association between miR-490-3p and HDAC2 was analyzed by luciferase activity reporter assay and Western blot assay.
RESULTS
We demonstrated that miR-490-3p functioned as a tumor-suppressive role in PCa progression. We found miR-490-3p expression was decreased in PCa cell lines. Down-regulation of miR-490-3p promoted the growth, migration, invasion but inhibited apoptosis of PCa cells. HDAC2 was validated as a direct target of miR-490-3p and promoted the progression of PCa cells. Further studies showed that HDAC2 could reverse the effects of miR-490-3p on growth, migration, invasion and apoptosis of PCa cells.
CONCLUSIONS
Our data highlighted the key role of miR-490-3p in the progression of PCa. Thus, miR-490-3p may be a novel cancer-specific therapeutic.
目的
微小 RNA（miRNA）被认为是人类癌症进展的关键参与者，包括前列腺癌（PCa），并有可能成为癌症治疗的靶点。在此，我们验证了一种肿瘤抑制 miRNA，miR-490-3p，它可能抑制 PCa 的进展。组蛋白去乙酰化酶 2（HDAC2）是一种在多种癌症中异常表达的蛋白质。然而，HDAC2 在 PCa 进展中的作用尚未完全阐明。
材料和方法
研究了 miR-490-3p 和 HDAC2 在 PCa 中的表达。分析了 miR-490-3p 或 HDAC2 表达对 PCa 细胞行为的影响。通过荧光素酶活性报告基因检测和 Western blot 检测分析 miR-490-3p 和 HDAC2 之间的关联。
结果
我们证明 miR-490-3p 在 PCa 进展中发挥肿瘤抑制作用。我们发现 miR-490-3p 在 PCa 细胞系中的表达降低。下调 miR-490-3p 促进了 PCa 细胞的生长、迁移、侵袭，但抑制了其凋亡。HDAC2 被验证为 miR-490-3p 的直接靶标，并促进了 PCa 细胞的进展。进一步的研究表明，HDAC2 可以逆转 miR-490-3p 对 PCa 细胞生长、迁移、侵袭和凋亡的影响。
结论
我们的数据强调了 miR-490-3p 在 PCa 进展中的关键作用。因此，miR-490-3p 可能是一种新的癌症特异性治疗方法。
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