Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering , Jiangxi Agricultural University , Nanchang 330045 , China.
J Agric Food Chem. 2019 Jul 31;67(30):8332-8338. doi: 10.1021/acs.jafc.9b02569. Epub 2019 Jul 18.
The excretion, tissue distribution, and metabolic profile of astilbin in rat were studied by HPLC and UPLC-QTOF-MS. Astilbin underwent isomerization in the small intestine, and its four isomers were found in feces. Besides, taxifolin, the aglycone of astilbin, and its further metabolites by gut microbes through hydrogenation, dehydration, and ring-fission were found. The total feces excretion of astilbin was about 14.4% of administration. The forming of zein-caseinate nanoparticles can significantly delay and reduce the feces excretion of astilbin. Astilbin and its isomers were absorbed in their intact form. The main metabolites found in plasma and tissues were the methylated products. Astilbin was rapidly distributed in various tissues including brain and maintained relatively high concentration in heart. Compared with other tissues, significantly higher concentration and longer duration of astilbin were found in the gastrointestinal tract. Astilbin and its isomers were excreted in their intact and methylated form in urine.
采用 HPLC 和 UPLC-QTOF-MS 研究了紫云英苷在大鼠体内的排泄、组织分布和代谢特征。紫云英苷在小肠中发生异构化，其 4 种异构体在粪便中被发现。此外，还发现了紫云英苷的苷元荭草苷以及肠道微生物通过加氢、脱水和环裂解生成的进一步代谢产物。紫云英苷的总粪便排泄量约为给药量的 14.4%。玉米醇溶蛋白-酪蛋白纳米粒的形成能显著延缓和减少紫云英苷的粪便排泄。紫云英苷及其异构体以完整形式被吸收。在血浆和组织中发现的主要代谢产物为甲基化产物。紫云英苷在包括脑在内的各种组织中迅速分布，并在心、肝、脾、肺和肾中维持相对较高的浓度。与其他组织相比，在胃肠道中发现了更高浓度和更长时间的紫云英苷和其异构体。紫云英苷及其异构体以完整和甲基化形式从尿液中排泄。
