Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
Department of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
J Biophotonics. 2019 Dec;12(12):e201900024. doi: 10.1002/jbio.201900024. Epub 2019 Jul 23.
The photothermal cancer therapy using cationic gold nanorods (GNRs) stabilized by quaternary ammonium salts (QAS) have a great potential to enhance conventional cancer treatment as it promises the effective eradication of cancer cells including cells resistant to radio- and chemo-therapy and the stimulation of anti-tumor immune response. However, as the cytotoxicity of the conventional alkanethiol-QAS compounds limits their utility in medicine, here we developed GNRs modified by novel highly hydrophilic cationic surfactant composed of the quaternary ammonium group and ethylene glycol chain N,N,N-trimethyl-3,6,9,12,15-pentaoxaheptadecyl-17-sulfanyl-1-ammonium bromide (POSAB) showing insignificant cytotoxicity in the free state. Surface modification of GNRs by POSAB allowed to prepare nanoparticles with good stability in water, high cellular uptake and localization in lysosomes that are a promising alternative to alkanethiol-stabilized GNRs especially for biomedical applications.
采用季铵盐（QAS）稳定的阳离子金纳米棒（GNRs）的光热癌症疗法具有增强传统癌症治疗的巨大潜力，因为它有望有效根除包括对放射和化学疗法有抗性的细胞以及刺激抗肿瘤免疫反应的细胞。然而，由于传统烷硫醇-QAS 化合物的细胞毒性限制了它们在医学中的应用，在这里，我们开发了由新型高度亲水阳离子表面活性剂修饰的 GNRs，该表面活性剂由季铵基团和乙二醇链 N，N，N-三甲基-3，6，9，12，15-五氧杂十七烷基-17-巯基-1-铵溴化物（POSAB）组成，在游离状态下表现出微不足道的细胞毒性。通过 POSAB 对 GNRs 的表面修饰，允许在水中制备具有良好稳定性的纳米颗粒，高细胞摄取和溶酶体定位，这是烷硫醇稳定的 GNRs 的有前途的替代品，特别是对于生物医学应用。
