Sivtseva L D, Levina L A
Zh Mikrobiol Epidemiol Immunobiol. 1988 Jan(1):11-5.
Sonicated lysates of 5 N. meningitidis strains, serogroup B, obtained from two solid serum-free culture media (a medium with casamino acids and a medium with Hottinger's hydrolysate) were studied with the aim of comparing the capacity of different group B meningococcal strains for the accumulation of group-specific polysaccharide. In the lysates obtained after 7-hour growth no sialic acid was found. After 20-hour cultivation, group-specific polysaccharide was detected in the lysates obtained from 4 out of 5 strains. All sonicated lysates obtained in these experiments were serologically active. The lysates obtained from the medium containing casamino acid had a higher content of group-specific polysaccharide. N. meningitidis strain 125, obtained at the Mechnikov Central Research Institute for Vaccines and Sera (Moscow) by selection, showed the highest content of capsular polysaccharide in microbial cells and the stable yield of biomass.
对从两种无血清固体培养基（一种含酪蛋白氨基酸的培养基和一种含霍廷格水解物的培养基）获得的5株B群脑膜炎奈瑟菌菌株的超声裂解物进行了研究，目的是比较不同B群脑膜炎球菌菌株积累群特异性多糖的能力。在7小时生长后获得的裂解物中未发现唾液酸。培养20小时后，在5株菌株中的4株获得的裂解物中检测到群特异性多糖。在这些实验中获得的所有超声裂解物都具有血清学活性。从含酪蛋白氨基酸的培养基中获得的裂解物中群特异性多糖的含量更高。在梅契尼科夫中央疫苗和血清研究所（莫斯科）通过筛选获得的脑膜炎奈瑟菌菌株125在微生物细胞中显示出最高的荚膜多糖含量和稳定的生物量产量。
