Reshilov L N, SAlov V F, Basnak'ian I A, Karabak V I, Borovkova V M
Zh Mikrobiol Epidemiol Immunobiol. 1989 May(5):20-6.
The formation of the rutheniophilic microcapsule was studied in 5 meningococcal strains, group B, in accordance with the data of transmission electron microscopy. The microcapsular group-specific meningococcal polysaccharide was shown to enter into a specific osmiophilic reaction with ruthenium red. The study revealed that the formation of the microcapsule was practically independent of the concentration of serum, ferric chloride and amino-nitrogen in the medium in the ranges used in the experiments (0-20%, 0-32 mg/l and 49-160 mg% respectively). As noted in this study, the presence of serum in the medium led to the formation of the pseudocapsule on the surface of meningococci. The intensity of the formation of the microcapsule was shown to depend on the phenotypical properties of definite strains, on the phase of bacterial growth (it reached its maximum values at the exponential phase), as well as on the concentration of glucose in the medium.
根据透射电子显微镜的数据，对5株B群脑膜炎球菌中嗜钌微胶囊的形成进行了研究。结果表明，微胶囊群特异性脑膜炎球菌多糖与钌红发生特异性嗜锇反应。研究发现，在实验所用的培养基浓度范围内（血清浓度0 - 20%、氯化铁浓度0 - 32 mg/l、氨基氮浓度49 - 160 mg%），微胶囊的形成实际上与培养基中血清、氯化铁和氨基氮的浓度无关。如本研究中所述，培养基中血清的存在会导致脑膜炎球菌表面形成假胶囊。结果表明，微胶囊形成的强度取决于特定菌株的表型特性、细菌生长阶段（在指数期达到最大值）以及培养基中葡萄糖的浓度。
