Ershov I v, Ovsiannikova I G, Gervazieva V B
Zh Mikrobiol Epidemiol Immunobiol. 1988 Feb(2):91-5.
The sensitivity and specificity of an enzyme immunoassay (EIA) system for the determination of total IgE in humans increases if polystyrene plates are sensitized with swine gamma-globulin and the conjugate is prepared with the use of sheep gamma-globulin obtained from the commercial preparation of sheep antiserum to human IgE. Such system becomes even more specific and sensitive if sheep antibodies to human IgE, purified by affinity chromatography, are used both for the sensitization of polystyrene plates and for the preparation of the conjugate. This conjugate is necessary for the development of EIA systems intended for the determination of specific human IgE-antibodies to various allergens.
如果用猪γ球蛋白致敏聚苯乙烯板，并使用从商业化制备的羊抗人IgE血清中获得的羊γ球蛋白制备结合物，用于测定人总IgE的酶免疫分析（EIA）系统的敏感性和特异性会提高。如果将通过亲和层析纯化的羊抗人IgE抗体用于聚苯乙烯板的致敏和结合物的制备，这样的系统会变得更加特异和敏感。这种结合物对于开发用于测定针对各种过敏原的特异性人IgE抗体的EIA系统是必需的。
