Reverse enzyme immunoassay for the determination of Lolium perenne IgE antibodies.

A microplate enzyme-immunoassay technique for the detection of specific anti-Lolium perenne IgE antibodies is described. This method consists of coating polystyrene wells with goat antihuman IgE (epsilon chain specific) and late selecting the IgE from the patients serum in order to finally reveal the bound specific IgE by means of a crude L. perenne extract conjugated with peroxidase. The optimal conditions necessary to achieve maximum sensitivity and specificity using this system have been studied. After testing 98 sera, the results showed a 97.9% correlation between our technique and the RAST.