Central Unit for Animal Research and Animal Welfare Affairs (ZETT) of the Heinrich-Heine-University of Düsseldorf, Düsseldorf, Germany.
Düsseldorf University Hospital, Department of OB/GYN and REI (UniKiD), Düsseldorf, Germany.
PLoS One. 2019 Jul 12;14(7):e0219604. doi: 10.1371/journal.pone.0219604. eCollection 2019.
Energy balance is essential for all species. Ligand-receptor interactions mediate processes that regulate body activities like reproduction and metabolism based on the energy status. Such receptors are the heparan sulfate proteoglycans and specifically the family of syndecans. Therefore we investigated the differences of metabolic parameters of heterozygous Syndecan 1 mice (Sdc1+/-) with reduced expression of Sdc1 and the corresponding wild type mice. Sdc1+/- mice have a reduced body weight although they show increased leptin and decreased corticosterone levels. Furthermore, their food and water intake is increased. This is accompanied with less adipose tissue, smaller adipocytes and thus an increased density of adipocytes. For the detailed analysis of the metabolism the automated PhenoMaster system has been used, which allowed continuous and undisturbed recording of food and water intake, energy expenditure and movement. The reason for the lower body weight was the higher energy expenditure of these animals compared to controls. Additionally, female Sdc1+/- mice showed an increased locomotor activity. Referring to organs, the intestine in Sdc1+/- mice was heavier and longer, but no differences at the cellular level could be observed. These findings were independent of normal mating or vice versa embryo transfers of Sdc1+/- and wild type embryos in recipient females of the other genotype. Herein we showed that the reduced expression of Sdc1 led to an altered metabolism on fetal as well as on maternal side, which may play a role in the growth restriction observed in human pregnancy pathologies and in mice lacking Sdc1.
能量平衡对所有物种都是至关重要的。配体-受体相互作用介导了根据能量状态调节身体活动的过程，如繁殖和代谢。这些受体是硫酸乙酰肝素蛋白聚糖，特别是 syndecan 家族。因此，我们研究了表达减少的 Syndecan 1 杂合子（Sdc1+/-）小鼠与相应野生型小鼠代谢参数的差异。尽管 Sdc1+/- 小鼠的瘦素水平升高，皮质酮水平降低，但它们的体重却减轻了。此外，它们的食物和水摄入量增加。这伴随着更少的脂肪组织，更小的脂肪细胞，因此脂肪细胞的密度增加。为了详细分析代谢情况，使用了自动化 PhenoMaster 系统，该系统允许连续和不受干扰地记录食物和水的摄入量、能量消耗和运动。这些动物的体重较低是由于它们的能量消耗高于对照动物。此外，Sdc1+/- 雌性小鼠的运动活性增加。就器官而言，Sdc1+/- 小鼠的肠道更重、更长，但在细胞水平上没有观察到差异。这些发现与正常交配无关，或者反之亦然，将 Sdc1+/- 和野生型胚胎的胚胎转移到另一种基因型的受体雌性中。在这里，我们表明 Sdc1 的表达减少导致胎儿和母体代谢发生改变，这可能在人类妊娠病理和缺乏 Sdc1 的小鼠中观察到的生长受限中发挥作用。
