Salvadori Schafer Andressa, D Baldissera Matheus, Bagolin da Silva Cássia, Sorraila de Oliveira Juliana, Igor Magalhães de Matos Antônio Francisco, Lopes Dornelles Guilherme, Grando Thirssa Helena, Trevisan Gressler Lucas, Stefanello Simone, Santi Eduarda, Pelegrine Minho Alessandro, Rodrigues Daniele, F Souza Carine, L R Leal Marta, G Monteiro Silvia, T A Lopes Sonia, Melazzo de Andrade Cinthia
Laboratório de Análises Clínicas Veterinárias, Departamento de Clínica de Pequenos Animais, Universidade Federal de Santa Maria, Av. Roraima nº1000, Santa Maria, Brazil.
Laboratório de Parasitologia Veterinária, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Exp Parasitol. 2019 Sep;204:107726. doi: 10.1016/j.exppara.2019.107726. Epub 2019 Jul 9.
The aims of this study were to evaluate if the use of copper oxide wire particles, isolated or in association with closantel, in lambs infected with Haemonchus contortus enhances the anthelmintic efficacy of closantel, as well as to evaluate the effects of treatment in hepatic energy metabolism, inflammatory markers and hematological and biochemical tests. The lambs were randomly divided into five groups (6 animals each), as follows: uninfected animals (Control); animals infected with H. contortus (HC); infected and treated with closantel (HC + CL); infected and treated with copper oxide wire particles (HC + Cu); and infected and treated with closantel plus copper oxide wire particles (HC + CL + Cu). The animals of infected groups were infected orally with H. contortus (5,000 L3 -larvae) and on day 14 post infection (p.i) the treatments were initiated. The egg per gram of feces (EPG), butyrylcholinesterase (BuChE), myeloperoxidase (MPO), adenylate kinase (AK) and pyruvate kinase (PK) activities and hematological and biochemical tests were evaluated. Treatments with copper oxide (isolated and associated) were able to reduce the EPG count on days 28, 35, 42 and 49 p.i when compared to HC group, while closantel was able to reduce EPG only from day 35 p.i. Moreover, treatment with closantel (isolated or associated) was able to prevent the inhibition of hepatic AK and PK activities caused by H. contortus infection, which may contribute to efficient intracellular energetic communication in order to maintain the balance between cellular ATP consumption and production. Butyrylcholinesterase and MPO activities were higher in infected lambs compared to uninfected, while treated groups showed lower enzymatic activity compared to the group HC. The use of all therapeutic protocols was able to reduce the EPG count. Based on these evidences, the use of copper oxide plus closantel may be considered an alternative to treat lambs infected by H. contortus.
本研究的目的是评估在感染捻转血矛线虫的羔羊中，单独使用或与氯氰碘柳胺联合使用氧化铜丝颗粒是否能增强氯氰碘柳胺的驱虫效果，以及评估治疗对肝脏能量代谢、炎症标志物以及血液学和生化检测的影响。羔羊被随机分为五组（每组6只动物），如下：未感染动物（对照组）；感染捻转血矛线虫的动物（HC）；感染并接受氯氰碘柳胺治疗的动物（HC + CL）；感染并接受氧化铜丝颗粒治疗的动物（HC + Cu）；感染并接受氯氰碘柳胺加氧化铜丝颗粒治疗的动物（HC + CL + Cu）。感染组的动物经口感染捻转血矛线虫（5000条L3幼虫），在感染后第14天开始治疗。评估每克粪便中的虫卵数（EPG）、丁酰胆碱酯酶（BuChE）、髓过氧化物酶（MPO）、腺苷酸激酶（AK）和丙酮酸激酶（PK）活性以及血液学和生化检测。与HC组相比，使用氧化铜（单独使用和联合使用）治疗能够在感染后第28、35、42和49天降低EPG计数，而氯氰碘柳胺仅从感染后第35天开始能够降低EPG。此外，使用氯氰碘柳胺（单独使用或联合使用）治疗能够预防捻转血矛线虫感染引起的肝脏AK和PK活性抑制，这可能有助于有效的细胞内能量通讯，以维持细胞ATP消耗和产生之间的平衡。与未感染的羔羊相比，感染羔羊的丁酰胆碱酯酶和MPO活性更高，而与HC组相比，治疗组的酶活性更低。所有治疗方案的使用都能够降低EPG计数。基于这些证据，氧化铜加氯氰碘柳胺的使用可被视为治疗感染捻转血矛线虫羔羊的一种替代方法。
