Campigotto Gabriela, Gebert Roger R, Santos Daiane S, Dos Reis João H, Alba Davi F, Cazarotto Chrystian J, Leal Marta L R, Baldissera Matheus D, Lopes Thalison F, Druzian Leticia T, Morsch Vera M, Vedovatto Marcelo, Da Silva Aleksandro S
Graduate Program in Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
Department of Animal Science, UDESC, Chapecó, SC, Brazil.
Exp Parasitol. 2019 Oct;205:107735. doi: 10.1016/j.exppara.2019.107735. Epub 2019 Aug 3.
Two experiments were performed to determine whether oral administration of copper oxide capsules controlled helminthic infections in Lacaune sheep without acute collateral effects on animal health. In experiment 1, 48 multiparous lactating sheep (60.1 ± 8.5 kg) were stratified according to initial number of eggs (Haemonchus contortus) per gram of feces (EPG) and were assigned randomly to 1 of two treatments (24 sheep/treatment): no oral administration (control) or oral administration of two copper capsules (treated; approximately 58 mg copper/kg body weight). Blood and fecal samples were collected on days 0, 15 and 45. Animals treated with copper capsules showed lower of EPG, eosinophils, acetylcholinesterase (AChE) in whole blood, and lower butyrylcholinesterase (BChE) activity in serum. Treated sheep had higher erythrocyte numbers, hemoglobin concentrations, hematocrit, and lymphocyte numbers. In experiment 2, 12 male lambs negative for helminths and coccidia were assigned randomly to one of two treatments (six lambs/treatment): control or treated (one copper capsule; approximately 58 mg copper/kg body weight); the experiment was designed to determine whether the results of experiment 1 were due to treatment or parasitism. Blood samples were collected on days 0, 5, 10 and 15 and fecal samples were collected on days 0, 7 and 15. Treated animals showed greater concentrations of lymphocytes; however, treatment had no effect on other hemogram variables, AChE and BChE activities, or levels of alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, creatinine, urea, albumin, total protein, and reactive oxygen species. These data suggest that copper capsules in dairy sheep efficiently controlled H. contortus infections. Treatment was not harmful to lambs during the first 15 days, i.e. there were no signs of acute toxicity.
进行了两项实验，以确定口服氧化铜胶囊是否能控制拉库内绵羊的蠕虫感染，同时对动物健康无急性附带影响。在实验1中，48只经产泌乳绵羊（体重60.1±8.5千克）根据每克粪便中（捻转血矛线虫）虫卵的初始数量进行分层，并随机分配到两种处理中的一种（每种处理24只绵羊）：不口服（对照）或口服两粒铜胶囊（处理组；约58毫克铜/千克体重）。在第0、15和45天采集血液和粪便样本。用铜胶囊处理的动物粪便中每克虫卵数、嗜酸性粒细胞、全血中的乙酰胆碱酯酶（AChE）较低，血清中的丁酰胆碱酯酶（BChE）活性也较低。处理过的绵羊红细胞数量、血红蛋白浓度、血细胞比容和淋巴细胞数量较高。在实验2中，12只对蠕虫和球虫呈阴性的雄性羔羊被随机分配到两种处理中的一种（每种处理6只羔羊）：对照或处理（一粒铜胶囊；约58毫克铜/千克体重）；该实验旨在确定实验1的结果是由于处理还是寄生虫感染所致。在第0、5、10和15天采集血液样本，在第0、7和15天采集粪便样本。处理过的动物淋巴细胞浓度更高；然而，处理对其他血常规变量、AChE和BChE活性，或丙氨酸转氨酶、天冬氨酸转氨酶、γ-谷氨酰转移酶、肌酐、尿素、白蛋白、总蛋白和活性氧水平没有影响。这些数据表明，奶牛口服铜胶囊可有效控制捻转血矛线虫感染。在最初15天内，处理对羔羊无害，即没有急性毒性迹象。
