Centre for Biosciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, 151001, India.
Department of Plant Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, 151001, India.
Plant Physiol Biochem. 2019 Sep;142:179-192. doi: 10.1016/j.plaphy.2019.07.006. Epub 2019 Jul 3.
Soil micro-biota plays a vital role in maintaining plant growth and fitness under normal and adverse conditions. Pseudomonas is one of the most important free-living and copious genera in south-west Punjab and involved in plant growth promotion under heavy metal stress. In this study, we have studied microbial diversity of the agricultural and marginal land based on 16S rRNA gene and screened eight strains of Pseudomonas for its tolerances towards various heavy metals and for plant growth promoting properties (PGP). The best strain is tested in chickpea plants against Arsenic (As) stress. All the strains responded differently to heavy metals viz. Arsenic, (As (0.3-0.5M) and As (250 μg mL) Cadmium (Cd) (250-350 μg mL), Chromium (Cr) (200-350 μg mL) and Mercury (Hg) (1-2 μg mL). Out of eight strains, only two strains (KM594398 and KM594397) showed plant growth promoting characters, concurrently they were highly tolerant to Arsenic (As). Pseudomonas citronellolis (PC) (KM594397) showed the best results in terms of As tolerance and plant growth promoting activity, hence further tested for actual plant growth response in chickpea (Cicer arietinum L.) under As (10-160 mg kg) stress. Pseudomonas citronellolis enhanced plant growth and dry biomass under As stress. High As tolerance and plant growth promoting activity of Pseudomonas citronellolis in chickpea especially designate this strain suitable for marginal lands and heavy metals contaminated sites.
土壤微生物在维持植物在正常和逆境条件下的生长和适应性方面起着至关重要的作用。假单胞菌是旁遮普西南部最重要的自由生活和丰富的属之一，在重金属胁迫下参与植物生长促进。在这项研究中，我们基于 16S rRNA 基因研究了农业和边缘土地的微生物多样性，并筛选了 8 株假单胞菌，以研究其对各种重金属的耐受性和植物生长促进特性（PGP）。最好的菌株在鹰嘴豆植物中针对砷（As）胁迫进行了测试。所有菌株对重金属的反应不同，例如砷（As（0.3-0.5M）和 As（250μgmL），镉（Cd）（250-350μgmL），铬（Cr）（200-350μgmL）和汞（Hg）（1-2μgmL）。在 8 株菌株中，只有 2 株菌株（KM594398 和 KM594397）表现出植物生长促进特性，同时它们对砷（As）具有高度耐受性。柠檬假单胞菌（PC）（KM594397）在砷（As）耐受性和植物生长促进活性方面表现出最佳效果，因此进一步在 10-160mgkg 的砷（As）胁迫下测试了其在鹰嘴豆（Cicer arietinum L.）中的实际植物生长反应。柠檬假单胞菌在砷胁迫下增强了植物生长和干生物量。柠檬假单胞菌在鹰嘴豆中具有高砷耐受性和植物生长促进活性，特别是指定该菌株适合边缘土地和重金属污染场地。
