Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, P. O. Box 577, Jhang Road, Faisalabad, Pakistan.
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, P. O. Box 577, Jhang Road, Faisalabad, Pakistan.
Curr Microbiol. 2019 Oct;76(10):1207-1214. doi: 10.1007/s00284-019-01737-1. Epub 2019 Jul 12.
Rhodococcus sp. Eu-32 has shown an extended novel dibenzothiophene desulfurization sulfur-specific 4S pathway and could remove significant amounts of organic sulfur from coal. Here, we present the draft genome sequence of Eu-32 with a genome size of approximately 5.61 Mb, containing 5065 protein coding sequences with a G+C content of 65.1%. The Rhodococcus sp. Eu-32 showed ~ 99% identity at the 16S rRNA gene sequence level while < 34% digital DNA-DNA hybridization and < 81% average nucleotide identity values with the genome sequence of most closely related known Rhodococcus species, suggesting that it is taxonomically different from the already reported Rhodococcus species. Among the annotated genes, 90 are involved in the metabolism of sulfur. Comparative genome analysis suggests many commonalities in sulfur metabolism gene sets that may have evolved due to many factors including ecological pressures. Our study and the genome sequence data will be available for further research and will provide insights into potential biotechnological and industrial applications of this bacterium.
硫杆菌属 Eu-32 具有一种扩展的新颖二苯并噻吩脱硫硫特异性 4S 途径，可从煤中去除大量有机硫。在这里，我们呈现 Eu-32 的草图基因组序列，其基因组大小约为 5.61Mb，包含 5065 个蛋白质编码序列，GC 含量为 65.1%。硫杆菌属 Eu-32 在 16S rRNA 基因序列水平上显示出约 99%的同一性，而与最密切相关的已知 Rhodococcus 种的基因组序列的数字 DNA-DNA 杂交值<34%，平均核苷酸同一性值<81%，表明其在分类上与已报道的 Rhodococcus 种不同。在注释的基因中，有 90 个参与硫代谢。比较基因组分析表明，硫代谢基因集有许多共同之处，这些共同之处可能是由于许多因素（包括生态压力）进化而来的。我们的研究和基因组序列数据将可供进一步研究，并为该细菌的潜在生物技术和工业应用提供见解。
